Detailed information of TA system TA116348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 114296..115020
• Replicon: plasmid pPvSC3
• Accession: NZ_CP034667
• Organism: Proteus vulgaris strain PvSC3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8I2DDP5
• Locus tag: EKQ45_RS00705
• Protein ID: WP_042847414.1
• Coordinates: 114709..115020 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EKQ45_RS00700
• Protein ID: WP_042847042.1
• Coordinates: 114296..114712 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKQ45_RS00655	109468..109686	+	219	WP_191901269.1	hypothetical protein	-
EKQ45_RS00660	109853..110413	+	561	WP_042847048.1	hypothetical protein	-
EKQ45_RS00665	110553..111122	+	570	WP_072035184.1	DUF459 domain-containing protein	-
EKQ45_RS00670	111221..111439	+	219	WP_042847047.1	hypothetical protein	-
EKQ45_RS00675	111563..112051	+	489	WP_042847046.1	hypothetical protein	-
EKQ45_RS00680	112109..112555	+	447	WP_052219361.1	hypothetical protein	-
EKQ45_RS00685	112619..113419	+	801	WP_042847045.1	methyltransferase	-
EKQ45_RS00690	113550..113885	+	336	WP_166268283.1	hypothetical protein	-
EKQ45_RS00695	113910..114218	+	309	WP_166268285.1	hypothetical protein	-
EKQ45_RS00700	114296..114712	-	417	WP_042847042.1	helix-turn-helix domain-containing protein	Antitoxin
EKQ45_RS00705	114709..115020	-	312	WP_042847414.1	type II toxin-antitoxin system HigB family toxin	Toxin
EKQ45_RS00710	115190..115756	+	567	WP_042847041.1	hypothetical protein	-
EKQ45_RS00715	115876..116193	+	318	WP_042847040.1	hypothetical protein	-
EKQ45_RS00720	116190..116495	+	306	WP_052219360.1	hypothetical protein	-
EKQ45_RS00725	116796..117671	+	876	WP_162762191.1	repA protein	-
EKQ45_RS00730	118187..118675	+	489	WP_042847408.1	hypothetical protein	-
EKQ45_RS00735	118738..119169	+	432	WP_144138807.1	hypothetical protein	-
EKQ45_RS00740	119355..119837	+	483	WP_042847038.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / floR / ant(2'')-Ia / catB8 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / tet(B) / cfr	-	1..284528	284528

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12448.21 Da
• Isoelectric Point: 10.2553

>T116348 WP_042847414.1 NZ_CP034667:c115020-114709 [Proteus vulgaris]
MHVISREPFDQASKRFPNSAQALSDIYRTLKRDSYQTPDELKKVFPSLDRMKYREKWWVIDISGNSLRMMFFADFDRGKI
FVKHITTHAEYDRLTDHYRRTKA

Nucleotide

• Length: 312 bp

>T116348 NZ_CP034667:c115020-114709 [Proteus vulgaris]
ATGCACGTAATATCAAGAGAACCTTTTGACCAGGCATCCAAGCGATTTCCAAACAGTGCTCAGGCGCTATCTGACATATA
TCGAACACTCAAGCGCGACAGTTATCAAACGCCTGATGAGCTTAAAAAGGTTTTCCCCAGCCTTGATCGGATGAAGTATA
GAGAGAAATGGTGGGTCATCGACATCAGTGGCAATTCATTAAGAATGATGTTTTTTGCCGACTTTGACCGGGGGAAAATA
TTCGTGAAACATATAACAACCCACGCTGAATACGATAGGTTGACTGATCACTACCGGAGAACAAAAGCATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15491.49 Da
• Isoelectric Point: 4.6438

>AT116348 WP_042847042.1 NZ_CP034667:c114712-114296 [Proteus vulgaris]
MMFQDAVNAAQNLISIVPLLGNSHSREDYEKAVQLVEHLVENDPDNPLIDLICAKIDAYEKTAPEFAEFNERLAKSNGGV
AALRTLMDQYHLNTTDFQDELGSRSYVSRILNGERGLTLEHIKKLSARFNIPASIFID

Nucleotide

• Length: 417 bp

>AT116348 NZ_CP034667:c114712-114296 [Proteus vulgaris]
ATGATGTTTCAAGATGCAGTAAACGCAGCACAAAACCTGATAAGCATTGTGCCGTTATTGGGAAACAGCCATTCTCGCGA
AGACTATGAAAAAGCCGTTCAACTGGTTGAGCACCTTGTCGAAAATGACCCAGACAACCCGCTTATCGACTTGATTTGCG
CGAAGATTGATGCGTATGAAAAAACAGCGCCCGAATTTGCTGAATTTAACGAGCGCTTAGCAAAAAGCAATGGCGGTGTA
GCAGCATTGAGAACGCTGATGGATCAATACCACCTTAATACGACAGACTTTCAAGATGAGCTCGGTTCGCGCTCATACGT
TAGCCGCATCCTCAACGGTGAACGAGGATTAACACTGGAGCACATTAAAAAACTGTCTGCGCGTTTTAATATCCCTGCCT
CCATTTTCATAGACTAA