Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 14604..15331 | Replicon | plasmid unnamed |
| Accession | NZ_CP034659 | ||
| Organism | Escherichia coli strain ATCC 98082 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A663AUB1 |
| Locus tag | EKH78_RS22270 | Protein ID | WP_000558568.1 |
| Coordinates | 14604..14915 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | EKH78_RS22275 | Protein ID | WP_000990393.1 |
| Coordinates | 14912..15331 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EKH78_RS22240 (EKH78_22395) | 9959..10114 | - | 156 | WP_001352007.1 | type I toxin-antitoxin system toxin HokD | - |
| EKH78_RS22245 (EKH78_22400) | 10449..11294 | - | 846 | WP_024192401.1 | helix-turn-helix domain-containing protein | - |
| EKH78_RS22250 (EKH78_22405) | 11324..12124 | - | 801 | WP_001187875.1 | phage antirepressor KilAC domain-containing protein | - |
| EKH78_RS22255 (EKH78_22410) | 12288..13331 | - | 1044 | WP_000743159.1 | phage antirepressor Ant | - |
| EKH78_RS22260 (EKH78_22415) | 13328..13549 | - | 222 | WP_000245703.1 | host cell division inhibitor Icd-like protein | - |
| EKH78_RS22265 (EKH78_22425) | 13763..14467 | + | 705 | WP_009364894.1 | IS6-like element IS26 family transposase | - |
| EKH78_RS22270 (EKH78_22430) | 14604..14915 | + | 312 | WP_000558568.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| EKH78_RS22275 (EKH78_22435) | 14912..15331 | + | 420 | WP_000990393.1 | helix-turn-helix domain-containing protein | Antitoxin |
| EKH78_RS22280 (EKH78_22440) | 15368..16570 | - | 1203 | WP_000753106.1 | hypothetical protein | - |
| EKH78_RS22285 (EKH78_22445) | 16563..16892 | - | 330 | WP_000121260.1 | hypothetical protein | - |
| EKH78_RS22290 (EKH78_22450) | 16889..17548 | - | 660 | WP_000078540.1 | hypothetical protein | - |
| EKH78_RS22295 (EKH78_22460) | 17972..18472 | - | 501 | WP_202118964.1 | Ref family recombination enhancement nuclease | - |
| EKH78_RS22300 (EKH78_22465) | 18478..19182 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| EKH78_RS22305 (EKH78_22470) | 19245..19613 | + | 369 | Protein_22 | IS30 family transposase | - |
| EKH78_RS22310 (EKH78_22475) | 19626..19871 | - | 246 | Protein_23 | transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | mrkA / mrkB / mrkC / mrkD / mrkF | 1..454520 | 454520 | |
| - | flank | IS/Tn | - | - | 9101..9805 | 704 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12372.11 Da Isoelectric Point: 10.0935
>T116341 WP_000558568.1 NZ_CP034659:14604-14915 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
Download Length: 312 bp
>T116341 NZ_CP034659:14604-14915 [Escherichia coli]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGATAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGATAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15560.74 Da Isoelectric Point: 4.3786
>AT116341 WP_000990393.1 NZ_CP034659:14912-15331 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQYKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQYKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
Download Length: 420 bp
>AT116341 NZ_CP034659:14912-15331 [Escherichia coli]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGTACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGTACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|