Detailed information of TA system TA116281

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3844941..3845353
• Replicon: chromosome
• Accession: NZ_CP034595
• Organism: Escherichia coli strain WCHEC035053S1G0

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: DK245_RS18950
• Protein ID: WP_000132601.1
• Coordinates: 3845012..3845353 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3844941..3845017 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DK245_RS18940	3841804..3843393	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
DK245_RS18945	3843390..3844784	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
-	3844941..3845017	-	77	NuclAT_14	-	Antitoxin
-	3844941..3845017	-	77	NuclAT_14	-	Antitoxin
-	3844941..3845017	-	77	NuclAT_14	-	Antitoxin
-	3844941..3845017	-	77	NuclAT_14	-	Antitoxin
-	3844941..3845017	-	77	NuclAT_15	-	Antitoxin
-	3844941..3845017	-	77	NuclAT_15	-	Antitoxin
-	3844941..3845017	-	77	NuclAT_15	-	Antitoxin
-	3844941..3845017	-	77	NuclAT_15	-	Antitoxin
DK245_RS18950	3845012..3845353	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
DK245_RS18955	3845515..3846894	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
DK245_RS18960	3846894..3847940	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T116281 WP_000132601.1 NZ_CP034595:3845012-3845353 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T116281 NZ_CP034595:3845012-3845353 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT116281 NZ_CP034595:c3845017-3844941 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin