Detailed information of TA system TA116279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3530502..3531196
• Replicon: chromosome
• Accession: NZ_CP034595
• Organism: Escherichia coli strain WCHEC035053S1G0

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: DK245_RS17485
• Protein ID: WP_001263489.1
• Coordinates: 3530502..3530900 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: DK245_RS17490
• Protein ID: WP_000554758.1
• Coordinates: 3530903..3531196 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3526090..3526170	-	81	NuclAT_12	-	-
-	3526090..3526170	-	81	NuclAT_12	-	-
-	3526090..3526170	-	81	NuclAT_12	-	-
-	3526090..3526170	-	81	NuclAT_12	-	-
DK245_RS17460	3526766..3527224	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
DK245_RS17465	3527485..3528942	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
DK245_RS17470	3528999..3529520	-	522	Protein_3392	peptide chain release factor H	-
DK245_RS17475	3529516..3529722	-	207	Protein_3393	RtcB family protein	-
DK245_RS17480	3530040..3530492	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
DK245_RS17485	3530502..3530900	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DK245_RS17490	3530903..3531196	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DK245_RS17495	3531248..3532303	-	1056	WP_001226164.1	DNA polymerase IV	-
DK245_RS17500	3532374..3533159	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
DK245_RS17505	3533131..3534843	+	1713	Protein_3399	flagellar biosynthesis protein FlhA	-
DK245_RS17510	3535067..3535564	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T116279 WP_001263489.1 NZ_CP034595:c3530900-3530502 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T116279 NZ_CP034595:c3530900-3530502 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT116279 WP_000554758.1 NZ_CP034595:c3531196-3530903 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT116279 NZ_CP034595:c3531196-3530903 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3