Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1654828..1655330 Replicon chromosome
Accession NZ_CP034595
Organism Escherichia coli strain WCHEC035053S1G0

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DK245_RS07960 Protein ID WP_000767829.1
Coordinates 1655076..1655330 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DK245_RS07955 Protein ID WP_001259255.1
Coordinates 1654828..1655079 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DK245_RS07930 1650006..1651073 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
DK245_RS07935 1651073..1652143 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
DK245_RS07940 1652140..1653444 - 1305 WP_000009594.1 histidinol dehydrogenase -
DK245_RS07945 1653450..1654349 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
DK245_RS07950 1654495..1654545 - 51 WP_001364200.1 his operon leader peptide -
DK245_RS07955 1654828..1655079 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DK245_RS07960 1655076..1655330 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DK245_RS07965 1655413..1656237 + 825 WP_000754737.1 SDR family oxidoreductase -
DK245_RS07970 1656283..1657212 + 930 WP_000803351.1 LysR family transcriptional regulator -
DK245_RS07975 1657427..1657489 + 63 WP_010723108.1 membrane protein YoeI -
DK245_RS07980 1657479..1658837 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DK245_RS07985 1659016..1660074 + 1059 WP_000492339.1 transport protein YeeE -
DK245_RS07990 1660088..1660315 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T116265 WP_000767829.1 NZ_CP034595:1655076-1655330 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T116265 NZ_CP034595:1655076-1655330 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT116265 WP_001259255.1 NZ_CP034595:1654828-1655079 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT116265 NZ_CP034595:1654828-1655079 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References