Detailed information of TA system TA116256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1131404..1132065
• Replicon: chromosome
• Accession: NZ_CP034593
• Organism: Flaviflexus sp. H23T48

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: EJ997_RS05085
• Protein ID: WP_126703614.1
• Coordinates: 1131715..1132065 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: EJ997_RS05080
• Protein ID: WP_126703613.1
• Coordinates: 1131404..1131709 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ997_RS05055	1127231..1128907	+	1677	WP_126703608.1	GAF domain-containing protein	-
EJ997_RS05060	1129042..1129269	-	228	WP_126703609.1	hypothetical protein	-
EJ997_RS05065	1129520..1129912	-	393	WP_126703610.1	VOC family protein	-
EJ997_RS05070	1130092..1130742	-	651	WP_126703611.1	response regulator transcription factor	-
EJ997_RS05075	1130916..1131164	-	249	WP_126703612.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EJ997_RS05080	1131404..1131709	-	306	WP_126703613.1	helix-turn-helix domain-containing protein	Antitoxin
EJ997_RS05085	1131715..1132065	-	351	WP_126703614.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJ997_RS05090	1132259..1133143	-	885	WP_126703615.1	bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase	-
EJ997_RS05095	1133136..1133867	-	732	WP_126703616.1	ribonuclease III	-
EJ997_RS05100	1133812..1134417	-	606	WP_164719807.1	DUF177 domain-containing protein	-
EJ997_RS05105	1134487..1134930	-	444	WP_126703618.1	DNA starvation/stationary phase protection protein	-
EJ997_RS05110	1135306..1136064	-	759	WP_126703619.1	hypothetical protein	-
EJ997_RS05115	1136057..1136536	-	480	WP_126703620.1	pantetheine-phosphate adenylyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (11-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-84)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13171.15 Da
• Isoelectric Point: 10.5339

>T116256 WP_126703614.1 NZ_CP034593:c1132065-1131715 [Flaviflexus sp. H23T48]
MWSVDLKLVSDWLTGLDQRSQVQVVAAIELLQDVGPQLGRPIVDTVTGSRHKNMKELRPGSSGRSELRILFAFDPARRAI
FLVAGDKSGNWKSWYRKNIPLADDLFDEHLRKQKGK

Nucleotide

• Length: 351 bp

>T116256 NZ_CP034593:c1132065-1131715 [Flaviflexus sp. H23T48]
ATGTGGAGCGTCGATCTCAAACTCGTCTCCGATTGGCTGACTGGTCTAGATCAGCGCTCACAGGTTCAGGTCGTTGCTGC
GATTGAGTTGCTCCAAGATGTTGGTCCACAGCTCGGCCGCCCAATTGTCGACACGGTAACGGGGTCGCGGCACAAGAACA
TGAAAGAGTTGCGTCCCGGCTCATCCGGTCGATCAGAGCTCCGGATCTTATTCGCATTCGATCCGGCAAGGCGAGCGATC
TTCCTGGTTGCAGGTGACAAATCTGGAAACTGGAAGAGTTGGTATCGGAAGAATATTCCGCTGGCTGACGATCTCTTTGA
CGAACACCTACGAAAGCAGAAAGGTAAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11314.87 Da
• Isoelectric Point: 5.0026

>AT116256 WP_126703613.1 NZ_CP034593:c1131709-1131404 [Flaviflexus sp. H23T48]
MSVSLEDFLAENPVDREAVEKEKARMLAEVRAFRLRELREKAGLTQAEVADRIGVGQRQVSKIEHGDLETARIGTIRRYV
EAVGGELTVEFISGDDRMKVA

Nucleotide

• Length: 306 bp

>AT116256 NZ_CP034593:c1131709-1131404 [Flaviflexus sp. H23T48]
ATGTCTGTAAGTCTTGAAGATTTTCTCGCGGAGAATCCGGTTGATCGTGAAGCCGTTGAAAAAGAAAAGGCACGCATGCT
TGCAGAGGTTCGTGCTTTTCGACTCCGTGAGCTCCGTGAGAAAGCGGGACTAACGCAAGCCGAGGTCGCGGATCGCATCG
GTGTTGGGCAACGGCAGGTCTCGAAGATCGAACACGGCGATCTGGAAACTGCCAGAATCGGCACGATTCGTCGTTACGTA
GAAGCCGTTGGCGGTGAACTTACCGTTGAGTTCATCAGCGGTGACGATCGCATGAAGGTCGCATAG