Detailed information of TA system TA116252

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 48091..48361
• Replicon: plasmid pL37-4
• Accession: NZ_CP034592
• Organism: Escherichia coli strain L37

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EKM58_RS23535
• Protein ID: WP_001312861.1
• Coordinates: 48203..48361 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	48091..48154 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKM58_RS23510	43802..44329	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
EKM58_RS23515	44387..44620	+	234	WP_000006003.1	DUF905 family protein	-
EKM58_RS23520	44681..46704	+	2024	Protein_46	ParB/RepB/Spo0J family partition protein	-
EKM58_RS23525	46773..47207	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EKM58_RS23530	47204..47923	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	47935..48159	+	225	NuclAT_0	-	-
-	47935..48159	+	225	NuclAT_0	-	-
-	47935..48159	+	225	NuclAT_0	-	-
-	47935..48159	+	225	NuclAT_0	-	-
EKM58_RS23920	47944..48123	-	180	WP_001309233.1	hypothetical protein	-
-	48091..48154	-	64	-	-	Antitoxin
EKM58_RS23535	48203..48361	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
EKM58_RS24025	48599..48976	-	378	Protein_51	hypothetical protein	-
EKM58_RS23555	49276..49572	+	297	WP_001272251.1	hypothetical protein	-
EKM58_RS23560	49683..50504	+	822	WP_001234445.1	DUF945 domain-containing protein	-
EKM58_RS23565	50801..51448	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
EKM58_RS23570	51725..52108	+	384	WP_000124981.1	relaxosome protein TraM	-
EKM58_RS23575	52299..52985	+	687	WP_015059009.1	PAS domain-containing protein	-
EKM58_RS23580	53079..53306	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / blaTEM-1B / blaCTX-M-55	-	1..76559	76559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T116252 WP_001312861.1 NZ_CP034592:48203-48361 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T116252 NZ_CP034592:48203-48361 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT116252 NZ_CP034592:c48154-48091 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin