Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2950359..2950861 Replicon chromosome
Accession NZ_CP034589
Organism Escherichia coli strain L37

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EKM58_RS14575 Protein ID WP_000767829.1
Coordinates 2950359..2950613 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EKM58_RS14580 Protein ID WP_001259255.1
Coordinates 2950610..2950861 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKM58_RS14545 2945374..2945601 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EKM58_RS14550 2945615..2946673 - 1059 WP_000492340.1 transport protein YeeE -
EKM58_RS14555 2946852..2948210 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EKM58_RS14560 2948200..2948262 - 63 WP_010723108.1 membrane protein YoeI -
EKM58_RS14565 2948477..2949406 - 930 WP_000803360.1 LysR family transcriptional regulator -
EKM58_RS14570 2949452..2950276 - 825 WP_000754737.1 SDR family oxidoreductase -
EKM58_RS14575 2950359..2950613 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EKM58_RS14580 2950610..2950861 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EKM58_RS14585 2951144..2951194 + 51 WP_001364200.1 his operon leader peptide -
EKM58_RS14590 2951340..2952239 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EKM58_RS14595 2952245..2953549 + 1305 WP_001572810.1 histidinol dehydrogenase -
EKM58_RS14600 2953546..2954616 + 1071 WP_001572811.1 histidinol-phosphate transaminase -
EKM58_RS14605 2954613..2955680 + 1068 WP_001572812.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T116245 WP_000767829.1 NZ_CP034589:c2950613-2950359 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T116245 NZ_CP034589:c2950613-2950359 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT116245 WP_001259255.1 NZ_CP034589:c2950861-2950610 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT116245 NZ_CP034589:c2950861-2950610 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References