Detailed information of TA system TA116230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 4628184..4628716
• Replicon: chromosome
• Accession: NZ_CP034587
• Organism: Streptomyces luteoverticillatus strain CGMCC 15060

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: EKH77_RS20680
• Protein ID: WP_126915826.1
• Coordinates: 4628462..4628716 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A3S9PLP0
• Locus tag: EKH77_RS20675
• Protein ID: WP_073443651.1
• Coordinates: 4628184..4628465 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKH77_RS20655	4624430..4625419	+	990	WP_126915822.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
EKH77_RS20660	4625450..4626214	+	765	WP_126915823.1	ROK family protein	-
EKH77_RS20665	4626111..4626710	-	600	WP_126915824.1	hypothetical protein	-
EKH77_RS20670	4626898..4627986	+	1089	WP_126915825.1	redox-regulated ATPase YchF	-
EKH77_RS20675	4628184..4628465	+	282	WP_073443651.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
EKH77_RS20680	4628462..4628716	+	255	WP_126915826.1	Txe/YoeB family addiction module toxin	Toxin
EKH77_RS20685	4628977..4629891	+	915	WP_126915827.1	hypothetical protein	-
EKH77_RS20690	4630227..4630808	-	582	WP_126915828.1	Uma2 family endonuclease	-
EKH77_RS20695	4630882..4633416	-	2535	WP_126915829.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4621310..4629891	8581

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-64)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10096.60 Da
• Isoelectric Point: 8.4461

>T116230 WP_126915826.1 NZ_CP034587:4628462-4628716 [Streptomyces luteoverticillatus]
VRLVFEDQGWEDYTSWLKNDRKMLARINKLIEDVRRDPFAGIGKPEPLKYHLPGAWSRRIDDEHRLVYLVTDKEIVILAA
RYHY

Nucleotide

• Length: 255 bp

>T116230 NZ_CP034587:4628462-4628716 [Streptomyces luteoverticillatus]
GTGAGGCTTGTCTTTGAGGACCAGGGCTGGGAGGACTACACGTCCTGGCTCAAGAACGACCGCAAGATGCTCGCCCGGAT
CAACAAGCTCATCGAGGACGTCAGGCGTGACCCCTTCGCGGGCATCGGCAAGCCGGAGCCGCTGAAGTATCACCTGCCTG
GGGCCTGGTCGCGACGGATCGACGACGAGCACCGCCTCGTCTATCTGGTGACAGATAAGGAGATCGTCATCCTCGCCGCC
CGGTATCACTACTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10099.37 Da
• Isoelectric Point: 5.6358

>AT116230 WP_073443651.1 NZ_CP034587:4628184-4628465 [Streptomyces luteoverticillatus]
MSITASEARKELFPLIKKVNENHEAIEIVSKHGNAVLVSAEDYAALREGSYLLRSPANARRLLKAYENALSHINVSEREL
IDPESPDAGAGAA

Nucleotide

• Length: 282 bp

>AT116230 NZ_CP034587:4628184-4628465 [Streptomyces luteoverticillatus]
ATGTCCATAACCGCGAGTGAAGCCCGCAAGGAACTGTTTCCGTTGATCAAAAAGGTCAACGAGAATCATGAGGCCATCGA
GATCGTCTCCAAGCACGGCAACGCCGTGCTTGTCTCAGCCGAAGACTACGCGGCGCTGCGCGAGGGCTCTTACCTGCTGC
GCTCGCCGGCGAACGCCCGGCGGCTGCTCAAGGCCTACGAGAACGCTCTGTCGCACATCAACGTGTCGGAGCGTGAGCTG
ATCGATCCCGAGTCACCGGACGCAGGTGCGGGTGCCGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S9PLP0