Detailed information of TA system TA116154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2700125..2700810
• Replicon: chromosome
• Accession: NZ_CP034483
• Organism: Rahnella aquatilis strain KM05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: H8NS19
• Locus tag: EJP80_RS12340
• Protein ID: WP_013575753.1
• Coordinates: 2700125..2700439 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EJP80_RS12345
• Protein ID: WP_015690029.1
• Coordinates: 2700493..2700810 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP80_RS12325	2696967..2697986	+	1020	WP_119261714.1	transporter	-
EJP80_RS12330	2698008..2699711	-	1704	WP_013575751.1	chloride channel protein	-
EJP80_RS12335	2699718..2699951	-	234	WP_013575752.1	hypothetical protein	-
EJP80_RS12340	2700125..2700439	+	315	WP_013575753.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJP80_RS12345	2700493..2700810	+	318	WP_015690029.1	HigA family addiction module antidote protein	Antitoxin
EJP80_RS12350	2700925..2702502	-	1578	WP_015690030.1	ABC transporter substrate-binding protein	-
EJP80_RS12355	2702645..2703322	-	678	WP_015690031.1	ATP-binding cassette domain-containing protein	-
EJP80_RS12360	2703315..2704199	-	885	WP_013575757.1	ABC transporter ATP-binding protein	-
EJP80_RS12365	2704189..2705067	-	879	WP_013575758.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12595.39 Da
• Isoelectric Point: 9.2702

>T116154 WP_013575753.1 NZ_CP034483:2700125-2700439 [Rahnella aquatilis]
MNDMLRISSFRDQWLEDFFHFATPHRKIPWTLATALERKLDMINAAMDYRDLLWPPGNRYEDLAPPLKGFSSIRVNEQYR
LIFYWREGKAHGLYLDPHGYRSYR

Nucleotide

• Length: 315 bp

>T116154 NZ_CP034483:2700125-2700439 [Rahnella aquatilis]
ATGAACGATATGCTCAGGATAAGCAGTTTCCGCGATCAATGGCTGGAAGACTTTTTCCACTTCGCCACTCCCCATCGCAA
AATACCCTGGACGCTGGCAACTGCTCTGGAAAGGAAGCTGGATATGATCAATGCCGCAATGGATTATCGCGATTTACTCT
GGCCCCCGGGCAATCGCTATGAAGATCTGGCCCCCCCGTTAAAGGGATTTTCATCGATCAGGGTGAATGAGCAGTACCGT
CTGATTTTTTACTGGCGGGAAGGAAAAGCACATGGCCTGTATTTAGATCCCCACGGTTACAGGAGTTACCGGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12115.02 Da
• Isoelectric Point: 8.6390

>AT116154 WP_015690029.1 NZ_CP034483:2700493-2700810 [Rahnella aquatilis]
MAVTTRKPATVGDILLYEFLEPAGLRINDLADMLNVHRNTISALVNNNRKLTVDIAFRLAKTFETSVEFWLNLQTNVDIW
EVRNNPRLMDDINKVAHLKDFMKKS

Nucleotide

• Length: 318 bp

>AT116154 NZ_CP034483:2700493-2700810 [Rahnella aquatilis]
ATGGCTGTGACCACACGTAAACCCGCTACCGTTGGTGATATTTTACTTTACGAATTTCTCGAGCCTGCAGGCCTGCGGAT
CAACGATCTCGCCGACATGCTGAATGTTCACCGCAATACCATCAGCGCGCTGGTGAATAACAACCGCAAACTGACCGTTG
ATATTGCCTTCCGGCTGGCAAAGACATTTGAGACCTCTGTCGAGTTTTGGTTAAACCTGCAAACCAATGTCGATATTTGG
GAAGTGCGCAATAACCCGCGCCTGATGGATGACATTAACAAAGTCGCCCATCTGAAAGATTTTATGAAAAAGTCATGA

Structures

Toxin

• AlphaFold DB: H8NS19