Detailed information of TA system TA116139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2641836..2642521
• Replicon: chromosome
• Accession: NZ_CP034482
• Organism: Rahnella aquatilis strain KM12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: H8NS19
• Locus tag: EJP81_RS12010
• Protein ID: WP_013575753.1
• Coordinates: 2641836..2642150 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EJP81_RS12015
• Protein ID: WP_015690029.1
• Coordinates: 2642204..2642521 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP81_RS11995	2638678..2639697	+	1020	WP_013575750.1	transporter	-
EJP81_RS12000	2639719..2641422	-	1704	WP_013575751.1	chloride channel protein	-
EJP81_RS12005	2641429..2641662	-	234	WP_013575752.1	hypothetical protein	-
EJP81_RS12010	2641836..2642150	+	315	WP_013575753.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJP81_RS12015	2642204..2642521	+	318	WP_015690029.1	HigA family addiction module antidote protein	Antitoxin
EJP81_RS12020	2642636..2644213	-	1578	WP_015690030.1	ABC transporter substrate-binding protein	-
EJP81_RS12025	2644356..2645033	-	678	WP_015690031.1	ATP-binding cassette domain-containing protein	-
EJP81_RS12030	2645026..2645910	-	885	WP_013575757.1	ABC transporter ATP-binding protein	-
EJP81_RS12035	2645900..2646778	-	879	WP_013575758.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12595.39 Da
• Isoelectric Point: 9.2702

>T116139 WP_013575753.1 NZ_CP034482:2641836-2642150 [Rahnella aquatilis]
MNDMLRISSFRDQWLEDFFHFATPHRKIPWTLATALERKLDMINAAMDYRDLLWPPGNRYEDLAPPLKGFSSIRVNEQYR
LIFYWREGKAHGLYLDPHGYRSYR

Nucleotide

• Length: 315 bp

>T116139 NZ_CP034482:2641836-2642150 [Rahnella aquatilis]
ATGAACGATATGCTCAGGATAAGCAGTTTCCGCGATCAATGGCTGGAAGACTTTTTCCACTTCGCCACTCCCCATCGCAA
AATACCCTGGACGCTGGCAACTGCTCTGGAAAGGAAGCTGGATATGATCAATGCCGCAATGGATTATCGCGATTTACTCT
GGCCCCCGGGCAATCGCTATGAAGATCTGGCCCCCCCGTTAAAGGGATTTTCATCGATCAGGGTGAATGAGCAGTACCGT
CTGATTTTTTACTGGCGGGAAGGAAAAGCACATGGCCTGTATTTAGATCCCCACGGTTACAGGAGTTACCGGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12115.02 Da
• Isoelectric Point: 8.6390

>AT116139 WP_015690029.1 NZ_CP034482:2642204-2642521 [Rahnella aquatilis]
MAVTTRKPATVGDILLYEFLEPAGLRINDLADMLNVHRNTISALVNNNRKLTVDIAFRLAKTFETSVEFWLNLQTNVDIW
EVRNNPRLMDDINKVAHLKDFMKKS

Nucleotide

• Length: 318 bp

>AT116139 NZ_CP034482:2642204-2642521 [Rahnella aquatilis]
ATGGCTGTGACCACACGTAAACCCGCTACCGTTGGTGATATTTTACTTTACGAATTTCTCGAGCCTGCAGGCCTGCGGAT
CAACGATCTCGCCGACATGCTGAATGTTCACCGCAATACCATCAGCGCGCTGGTGAATAACAACCGCAAACTGACCGTTG
ATATTGCCTTCCGGCTGGCAAAGACATTTGAGACCTCTGTCGAGTTTTGGTTAAACCTGCAAACCAATGTCGATATTTGG
GAAGTGCGCAATAACCCGCGCCTGATGGATGACATTAACAAAGTCGCCCATCTGAAAGATTTTATGAAAAAGTCATGA

Structures

Toxin

• AlphaFold DB: H8NS19