Detailed information of TA system TA116123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2641139..2641824
• Replicon: chromosome
• Accession: NZ_CP034481
• Organism: Rahnella aquatilis strain KM25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: H8NS19
• Locus tag: EJP79_RS12010
• Protein ID: WP_013575753.1
• Coordinates: 2641139..2641453 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EJP79_RS12015
• Protein ID: WP_015690029.1
• Coordinates: 2641507..2641824 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP79_RS11995	2637981..2639000	+	1020	WP_013575750.1	transporter	-
EJP79_RS12000	2639022..2640725	-	1704	WP_013575751.1	chloride channel protein	-
EJP79_RS12005	2640732..2640965	-	234	WP_013575752.1	hypothetical protein	-
EJP79_RS12010	2641139..2641453	+	315	WP_013575753.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJP79_RS12015	2641507..2641824	+	318	WP_015690029.1	HigA family addiction module antidote protein	Antitoxin
EJP79_RS12020	2641939..2643516	-	1578	WP_015690030.1	ABC transporter substrate-binding protein	-
EJP79_RS12025	2643659..2644336	-	678	WP_015690031.1	ATP-binding cassette domain-containing protein	-
EJP79_RS12030	2644329..2645213	-	885	WP_013575757.1	ABC transporter ATP-binding protein	-
EJP79_RS12035	2645203..2646081	-	879	WP_013575758.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-70)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12595.39 Da
• Isoelectric Point: 9.2702

>T116123 WP_013575753.1 NZ_CP034481:2641139-2641453 [Rahnella aquatilis]
MNDMLRISSFRDQWLEDFFHFATPHRKIPWTLATALERKLDMINAAMDYRDLLWPPGNRYEDLAPPLKGFSSIRVNEQYR
LIFYWREGKAHGLYLDPHGYRSYR

Nucleotide

• Length: 315 bp

>T116123 NZ_CP034481:2641139-2641453 [Rahnella aquatilis]
ATGAACGATATGCTCAGGATAAGCAGTTTCCGCGATCAATGGCTGGAAGACTTTTTCCACTTCGCCACTCCCCATCGCAA
AATACCCTGGACGCTGGCAACTGCTCTGGAAAGGAAGCTGGATATGATCAATGCCGCAATGGATTATCGCGATTTACTCT
GGCCCCCGGGCAATCGCTATGAAGATCTGGCCCCCCCGTTAAAGGGATTTTCATCGATCAGGGTGAATGAGCAGTACCGT
CTGATTTTTTACTGGCGGGAAGGAAAAGCACATGGCCTGTATTTAGATCCCCACGGTTACAGGAGTTACCGGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12115.02 Da
• Isoelectric Point: 8.6390

>AT116123 WP_015690029.1 NZ_CP034481:2641507-2641824 [Rahnella aquatilis]
MAVTTRKPATVGDILLYEFLEPAGLRINDLADMLNVHRNTISALVNNNRKLTVDIAFRLAKTFETSVEFWLNLQTNVDIW
EVRNNPRLMDDINKVAHLKDFMKKS

Nucleotide

• Length: 318 bp

>AT116123 NZ_CP034481:2641507-2641824 [Rahnella aquatilis]
ATGGCTGTGACCACACGTAAACCCGCTACCGTTGGTGATATTTTACTTTACGAATTTCTCGAGCCTGCAGGCCTGCGGAT
CAACGATCTCGCCGACATGCTGAATGTTCACCGCAATACCATCAGCGCGCTGGTGAATAACAACCGCAAACTGACCGTTG
ATATTGCCTTCCGGCTGGCAAAGACATTTGAGACCTCTGTCGAGTTTTGGTTAAACCTGCAAACCAATGTCGATATTTGG
GAAGTGCGCAATAACCCGCGCCTGATGGATGACATTAACAAAGTCGCCCATCTGAAAGATTTTATGAAAAAGTCATGA

Structures

Toxin

• AlphaFold DB: H8NS19