Detailed information of TA system TA116114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 425638..426468
• Replicon: plasmid pCFSAN047154_1
• Accession: NZ_CP034475
• Organism: Pantoea agglomerans strain CFSAN047154

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: D1629_RS21125
• Protein ID: WP_061703420.1
• Coordinates: 425638..426117 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A8X8DUC8
• Locus tag: D1629_RS21130
• Protein ID: WP_033773852.1
• Coordinates: 426124..426468 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1629_RS21115	420972..423374	+	2403	WP_061703422.1	maltodextrin phosphorylase	-
D1629_RS21120	423383..425458	+	2076	WP_061703421.1	4-alpha-glucanotransferase	-
D1629_RS21125	425638..426117	-	480	WP_061703420.1	type II toxin-antitoxin system YhaV family toxin	Toxin
D1629_RS21130	426124..426468	-	345	WP_033773852.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
D1629_RS21135	426566..426781	-	216	WP_061703419.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
D1629_RS21140	426970..427908	-	939	WP_061703418.1	maltose operon protein MalM	-
D1629_RS21145	428011..429312	-	1302	WP_033760237.1	maltoporin	-
D1629_RS21150	429356..430465	-	1110	WP_010247360.1	maltose/maltodextrin ABC transporter ATP-binding protein MalK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..613013	613013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-148)

Antitoxin

• PrlF antitoxin (15-113)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 18653.15 Da
• Isoelectric Point: 8.4504

>T116114 WP_061703420.1 NZ_CP034475:c426117-425638 [Pantoea agglomerans]
MEDSTVINGWHVYVHPCFESQLIALTNEVELLRAKYPESYQRKATTNLLAAVFKVINSEICADPHQAKFRQGDTLGEQNK
HWFRAKFLMQYRLFFRFSEQHKTIILAWMNDAETKRAYGSKRDAYKVFSGMLHNGYPPDDWALLLKQSQNWANTNSEYP

Nucleotide

• Length: 480 bp

>T116114 NZ_CP034475:c426117-425638 [Pantoea agglomerans]
ATGGAAGACAGCACGGTTATAAATGGATGGCACGTTTATGTTCATCCGTGTTTTGAATCGCAGCTGATTGCGCTGACCAA
TGAAGTGGAGTTACTCAGGGCTAAATATCCTGAAAGTTACCAGCGAAAAGCAACGACTAATCTTTTAGCTGCTGTGTTTA
AAGTTATCAATAGTGAGATTTGCGCCGATCCACATCAGGCAAAGTTTCGCCAGGGCGATACGCTGGGAGAACAAAATAAG
CATTGGTTCCGTGCAAAGTTTTTGATGCAGTATCGTTTGTTCTTTAGATTCAGCGAGCAACATAAAACCATCATCCTTGC
ATGGATGAATGACGCTGAGACCAAACGTGCTTATGGCAGTAAGCGTGACGCCTATAAAGTATTTTCAGGAATGTTGCATA
ACGGCTATCCACCTGACGACTGGGCATTGCTGTTAAAGCAATCTCAGAATTGGGCGAATACAAATTCGGAATATCCATAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12285.96 Da
• Isoelectric Point: 4.3278

>AT116114 WP_033773852.1 NZ_CP034475:c426468-426124 [Pantoea agglomerans]
MGTLTHANISLRASSKLTARSQTTIPAAVRDAMNLQPGENIEYAVLPGGKVLITRQETVTDDDPVMASFLSFLAADIRNT
PANIKTLDRGLLTRITSLTEGMDVDLDAPLMDDE

Nucleotide

• Length: 345 bp

>AT116114 NZ_CP034475:c426468-426124 [Pantoea agglomerans]
ATGGGAACTCTGACTCATGCAAACATCTCTCTGCGTGCTTCATCAAAACTGACAGCCCGCAGCCAGACCACTATCCCTGC
CGCAGTTCGTGATGCGATGAATCTGCAACCGGGTGAGAATATCGAATACGCTGTTCTGCCGGGCGGCAAAGTCCTTATTA
CCCGTCAGGAAACGGTGACTGATGATGATCCAGTGATGGCGAGCTTTCTGAGTTTTCTTGCTGCTGACATACGCAACACA
CCTGCAAATATTAAAACGCTGGATCGAGGATTATTAACTCGCATTACATCGTTGACCGAAGGTATGGACGTTGATTTGGA
TGCTCCACTCATGGACGATGAGTAA