Detailed information of TA system TA116103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 526090..526811
• Replicon: plasmid pCFSAN047153_1
• Accession: NZ_CP034470
• Organism: Pantoea agglomerans strain CFSAN047153

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D1628_RS21680
• Protein ID: WP_033773376.1
• Coordinates: 526500..526811 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D1628_RS21675
• Protein ID: WP_010247064.1
• Coordinates: 526090..526503 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1628_RS21655	522480..523214	-	735	WP_010247068.1	GntR family transcriptional regulator	-
D1628_RS21660	523791..524240	+	450	WP_033773372.1	PTS sugar transporter subunit IIA	-
D1628_RS21665	524289..524561	+	273	WP_010247066.1	PTS sugar transporter subunit IIB	-
D1628_RS21670	524601..525974	+	1374	WP_033773374.1	PTS ascorbate transporter subunit IIC	-
D1628_RS21675	526090..526503	-	414	WP_010247064.1	helix-turn-helix domain-containing protein	Antitoxin
D1628_RS21680	526500..526811	-	312	WP_033773376.1	type II toxin-antitoxin system HigB family toxin	Toxin
D1628_RS21685	527252..528805	+	1554	WP_129063350.1	aspartate aminotransferase family protein	-
D1628_RS21690	528816..530108	+	1293	WP_010247061.1	lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..613013	613013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12138.01 Da
• Isoelectric Point: 9.6067

>T116103 WP_033773376.1 NZ_CP034470:c526811-526500 [Pantoea agglomerans]
MHIISRGPFREAAIIYPNHASALDAAYLVLRDENFATPDALKISFQSLDRMKYREKWWVIDVGGNSLRIMFYADFDRGKI
FIKHIVTHAEYDKLVKKYRESKE

Nucleotide

• Length: 312 bp

>T116103 NZ_CP034470:c526811-526500 [Pantoea agglomerans]
ATGCATATCATCTCAAGAGGGCCGTTCAGGGAAGCGGCAATAATTTATCCAAATCATGCCTCCGCACTGGATGCGGCTTA
TCTGGTCCTAAGGGACGAAAACTTTGCCACGCCAGATGCGCTGAAAATTAGCTTTCAGAGCCTTGACCGCATGAAGTACC
GGGAAAAGTGGTGGGTAATCGATGTGGGCGGAAACAGCCTGAGAATCATGTTTTATGCTGACTTTGATCGCGGCAAAATT
TTTATCAAACACATCGTGACGCATGCGGAATATGACAAGCTGGTGAAGAAGTACCGGGAGAGTAAAGAATGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15402.41 Da
• Isoelectric Point: 4.9795

>AT116103 WP_010247064.1 NZ_CP034470:c526503-526090 [Pantoea agglomerans]
MITEAIQATNDLVRIVPFLGGSTDKRDYEQALELVEYLVEHQPDSPLVEILSDKVARYENSAPEFAAFNARTDAIPRGVA
LLRVIMDQHGLTQSSFADEIGQRSYVSRILRGDRPLTDKHKARLAARFNLPFEAFAE

Nucleotide

• Length: 414 bp

>AT116103 NZ_CP034470:c526503-526090 [Pantoea agglomerans]
ATGATTACCGAAGCAATCCAGGCGACCAATGACCTGGTCAGAATTGTTCCCTTTCTGGGAGGCAGTACCGATAAAAGGGA
CTACGAGCAGGCGCTGGAACTGGTTGAATATCTGGTTGAGCACCAGCCGGACAGCCCGTTAGTAGAGATACTGTCTGATA
AAGTCGCCCGATACGAAAACAGCGCCCCTGAGTTCGCTGCGTTTAACGCGCGTACTGATGCTATTCCGCGCGGAGTAGCA
CTTTTGCGAGTCATCATGGATCAGCACGGCCTGACTCAATCCTCTTTTGCGGATGAAATAGGGCAGCGCTCTTATGTCAG
TCGTATCCTGCGTGGTGATCGTCCTTTGACGGATAAACATAAAGCCCGACTGGCAGCGCGTTTTAACCTGCCGTTTGAAG
CATTCGCTGAATAA