Detailed information of TA system TA116102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 380309..381139
• Replicon: plasmid pCFSAN047153_1
• Accession: NZ_CP034470
• Organism: Pantoea agglomerans strain CFSAN047153

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: D1628_RS21030
• Protein ID: WP_061703420.1
• Coordinates: 380660..381139 (+)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A8X8DUC8
• Locus tag: D1628_RS21025
• Protein ID: WP_033773852.1
• Coordinates: 380309..380653 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1628_RS21005	376312..377421	+	1110	WP_010247360.1	maltose/maltodextrin ABC transporter ATP-binding protein MalK	-
D1628_RS21010	377465..378766	+	1302	WP_033760237.1	maltoporin	-
D1628_RS21015	378869..379807	+	939	WP_061703418.1	maltose operon protein MalM	-
D1628_RS21020	379996..380211	+	216	WP_061703419.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
D1628_RS21025	380309..380653	+	345	WP_033773852.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
D1628_RS21030	380660..381139	+	480	WP_061703420.1	type II toxin-antitoxin system YhaV family toxin	Toxin
D1628_RS21035	381319..383394	-	2076	WP_061703421.1	4-alpha-glucanotransferase	-
D1628_RS21040	383403..385805	-	2403	WP_061703422.1	maltodextrin phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..613013	613013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-148)

Antitoxin

• PrlF antitoxin (15-113)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 18653.15 Da
• Isoelectric Point: 8.4504

>T116102 WP_061703420.1 NZ_CP034470:380660-381139 [Pantoea agglomerans]
MEDSTVINGWHVYVHPCFESQLIALTNEVELLRAKYPESYQRKATTNLLAAVFKVINSEICADPHQAKFRQGDTLGEQNK
HWFRAKFLMQYRLFFRFSEQHKTIILAWMNDAETKRAYGSKRDAYKVFSGMLHNGYPPDDWALLLKQSQNWANTNSEYP

Nucleotide

• Length: 480 bp

>T116102 NZ_CP034470:380660-381139 [Pantoea agglomerans]
ATGGAAGACAGCACGGTTATAAATGGATGGCACGTTTATGTTCATCCGTGTTTTGAATCGCAGCTGATTGCGCTGACCAA
TGAAGTGGAGTTACTCAGGGCTAAATATCCTGAAAGTTACCAGCGAAAAGCAACGACTAATCTTTTAGCTGCTGTGTTTA
AAGTTATCAATAGTGAGATTTGCGCCGATCCACATCAGGCAAAGTTTCGCCAGGGCGATACGCTGGGAGAACAAAATAAG
CATTGGTTCCGTGCAAAGTTTTTGATGCAGTATCGTTTGTTCTTTAGATTCAGCGAGCAACATAAAACCATCATCCTTGC
ATGGATGAATGACGCTGAGACCAAACGTGCTTATGGCAGTAAGCGTGACGCCTATAAAGTATTTTCAGGAATGTTGCATA
ACGGCTATCCACCTGACGACTGGGCATTGCTGTTAAAGCAATCTCAGAATTGGGCGAATACAAATTCGGAATATCCATAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12285.96 Da
• Isoelectric Point: 4.3278

>AT116102 WP_033773852.1 NZ_CP034470:380309-380653 [Pantoea agglomerans]
MGTLTHANISLRASSKLTARSQTTIPAAVRDAMNLQPGENIEYAVLPGGKVLITRQETVTDDDPVMASFLSFLAADIRNT
PANIKTLDRGLLTRITSLTEGMDVDLDAPLMDDE

Nucleotide

• Length: 345 bp

>AT116102 NZ_CP034470:380309-380653 [Pantoea agglomerans]
ATGGGAACTCTGACTCATGCAAACATCTCTCTGCGTGCTTCATCAAAACTGACAGCCCGCAGCCAGACCACTATCCCTGC
CGCAGTTCGTGATGCGATGAATCTGCAACCGGGTGAGAATATCGAATACGCTGTTCTGCCGGGCGGCAAAGTCCTTATTA
CCCGTCAGGAAACGGTGACTGATGATGATCCAGTGATGGCGAGCTTTCTGAGTTTTCTTGCTGCTGACATACGCAACACA
CCTGCAAATATTAAAACGCTGGATCGAGGATTATTAACTCGCATTACATCGTTGACCGAAGGTATGGACGTTGATTTGGA
TGCTCCACTCATGGACGATGAGTAA