Detailed information of TA system TA116099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2545777..2546437
• Replicon: chromosome
• Accession: NZ_CP034469
• Organism: Pantoea agglomerans strain CFSAN047153

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A349IFT5
• Locus tag: D1628_RS12060
• Protein ID: WP_010670918.1
• Coordinates: 2546084..2546437 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D1628_RS12055
• Protein ID: WP_010247918.1
• Coordinates: 2545777..2546079 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1628_RS12035	2541616..2542641	-	1026	WP_170933484.1	ABC transporter permease	-
D1628_RS12040	2542665..2544149	-	1485	WP_129062993.1	sugar ABC transporter ATP-binding protein	-
D1628_RS12045	2544182..2545105	-	924	WP_010247907.1	sugar ABC transporter substrate-binding protein	-
D1628_RS12055	2545777..2546079	-	303	WP_010247918.1	XRE family transcriptional regulator	Antitoxin
D1628_RS12060	2546084..2546437	-	354	WP_010670918.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1628_RS12065	2546736..2547824	-	1089	WP_029519619.1	hypothetical protein	-
D1628_RS12070	2548220..2550382	+	2163	WP_129062994.1	ferric-rhodotorulic acid/ferric-coprogen receptor FhuE	-
D1628_RS12075	2550459..2550860	-	402	WP_010247930.1	hypothetical protein	-
D1628_RS12080	2550959..2551240	-	282	WP_010247933.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13649.71 Da
• Isoelectric Point: 8.4949

>T116099 WP_010670918.1 NZ_CP034469:c2546437-2546084 [Pantoea agglomerans]
VWMIKTTERFDRWFTLLNESDRACVLAALMVLREKGPGLSRPYADTLKGSVYINMKELRIQSRGDPIRAFFAFDPNRTAI
VLCAGNKVGNEKRFYQEMLPVADREFTHWLNSFKDEE

Nucleotide

• Length: 354 bp

>T116099 NZ_CP034469:c2546437-2546084 [Pantoea agglomerans]
GTGTGGATGATTAAAACGACGGAGAGGTTTGATCGCTGGTTTACCTTGCTTAATGAGAGCGATCGCGCCTGCGTACTGGC
AGCATTAATGGTGTTGCGCGAAAAAGGGCCGGGGTTGTCCAGGCCATACGCGGATACGCTAAAAGGCTCTGTTTATATCA
ACATGAAAGAGCTGCGTATTCAGAGTCGCGGCGATCCGATAAGGGCTTTCTTTGCTTTTGACCCAAACCGAACGGCGATT
GTGCTTTGTGCCGGAAATAAGGTGGGCAATGAAAAGCGTTTTTATCAGGAGATGCTTCCGGTTGCCGATCGGGAATTTAC
ACACTGGCTGAATAGTTTTAAAGATGAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10941.72 Da
• Isoelectric Point: 5.7251

>AT116099 WP_010247918.1 NZ_CP034469:c2546079-2545777 [Pantoea agglomerans]
MGRTLEQLIADEKPEVVADAQAMATDILLNIHLAELREKVQKTQVEMAKALGITQPTVAGMEKPGRDLKLSTLKRYIDAA
GGKLRLVVELPDGSHYGFEV

Nucleotide

• Length: 303 bp

>AT116099 NZ_CP034469:c2546079-2545777 [Pantoea agglomerans]
ATGGGCAGAACGCTTGAACAGCTTATTGCGGATGAAAAACCGGAAGTCGTTGCCGACGCACAAGCGATGGCGACGGATAT
CCTGCTCAACATTCACCTTGCCGAGCTGCGCGAGAAAGTGCAGAAAACACAGGTCGAAATGGCAAAGGCGCTGGGCATTA
CACAGCCTACCGTGGCGGGCATGGAGAAGCCGGGCCGCGACCTGAAGCTCTCCACGCTCAAGCGCTATATCGATGCGGCA
GGGGGTAAGCTGCGGCTGGTGGTGGAACTGCCGGACGGCTCGCACTACGGGTTTGAGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A349IFT5