Detailed information of TA system TA116016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6924439..6925040
• Replicon: chromosome
• Accession: NZ_CP034432
• Organism: Bradyrhizobium sp. LCT2

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q89QW2
• Locus tag: EI171_RS32975
• Protein ID: WP_011085796.1
• Coordinates: 6924439..6924720 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: EI171_RS32980
• Protein ID: WP_011085795.1
• Coordinates: 6924732..6925040 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EI171_RS32940	6919870..6920184	+	315	WP_161535801.1	hypothetical protein	-
EI171_RS32945	6920230..6920553	+	324	WP_011085802.1	antibiotic biosynthesis monooxygenase	-
EI171_RS32950	6920576..6921442	-	867	WP_161535802.1	helix-turn-helix transcriptional regulator	-
EI171_RS32955	6921622..6922566	+	945	WP_011085800.1	metallophosphoesterase	-
EI171_RS32960	6922592..6922927	+	336	WP_011085799.1	cupredoxin family copper-binding protein	-
EI171_RS32965	6923055..6923603	+	549	WP_028172321.1	sigma-70 family RNA polymerase sigma factor	-
EI171_RS32970	6923600..6924367	+	768	WP_011085797.1	anti-sigma factor	-
EI171_RS32975	6924439..6924720	+	282	WP_011085796.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EI171_RS32980	6924732..6925040	+	309	WP_011085795.1	HigA family addiction module antidote protein	Antitoxin
EI171_RS32985	6925049..6926440	-	1392	WP_011085794.1	aminobacteriohopanetriol synthase HpnO	-
EI171_RS32990	6926582..6927244	+	663	WP_028172324.1	DUF2147 domain-containing protein	-
EI171_RS32995	6927212..6929800	-	2589	WP_011085792.1	MMPL family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10528.99 Da
• Isoelectric Point: 9.7579

>T116016 WP_011085796.1 NZ_CP034432:6924439-6924720 [Bradyrhizobium sp. LCT2]
VIRTFRDKTTEAVFDGESPKGFPADLVKVARRKLRYLHAAGELGDLRAPPGNRLEALSGNRKGQHSIRINDQFRVCFIWT
PQGPVEVEIVDYH

Nucleotide

• Length: 282 bp

>T116016 NZ_CP034432:6924439-6924720 [Bradyrhizobium sp. LCT2]
GTGATCAGAACGTTTCGGGACAAAACGACTGAGGCAGTCTTCGACGGAGAAAGTCCCAAAGGCTTTCCAGCCGATCTCGT
GAAGGTTGCACGCCGGAAACTGCGTTATCTGCACGCTGCCGGCGAGCTCGGCGATCTAAGGGCGCCGCCCGGCAACCGTC
TGGAGGCCCTGAGCGGCAATCGCAAGGGACAGCACTCGATCAGGATCAATGATCAGTTCCGAGTGTGCTTCATCTGGACA
CCGCAAGGGCCCGTCGAGGTCGAGATTGTGGACTATCACTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11266.07 Da
• Isoelectric Point: 9.0966

>AT116016 WP_011085795.1 NZ_CP034432:6924732-6925040 [Bradyrhizobium sp. LCT2]
MAKKLKPMHPGEVLREEFLVPLNMSAGALAKTCGLPRTRIERIANEQTGITADTALRLAKALGTTAELWLNLQNDYDIQI
AKRDLGKALDRIETVNRPQAAE

Nucleotide

• Length: 309 bp

>AT116016 NZ_CP034432:6924732-6925040 [Bradyrhizobium sp. LCT2]
ATGGCCAAAAAACTCAAGCCCATGCACCCCGGTGAGGTTCTACGGGAGGAGTTTCTAGTCCCACTGAACATGTCCGCTGG
CGCGCTCGCCAAGACGTGCGGCTTGCCGCGCACGCGCATCGAGCGGATCGCGAATGAGCAGACCGGAATCACCGCCGACA
CCGCGCTTCGACTGGCCAAGGCGCTCGGCACAACGGCCGAACTCTGGCTCAATCTTCAGAATGATTATGACATCCAGATC
GCGAAGCGCGATCTCGGCAAGGCACTTGACCGCATCGAGACCGTGAACAGGCCGCAAGCGGCTGAATGA

Structures

Toxin

• AlphaFold DB: Q89QW2