Detailed information of TA system TA116015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5242023..5242618
• Replicon: chromosome
• Accession: NZ_CP034429
• Organism: Pseudomonas aeruginosa strain GIMC5015:PAKB6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: PAKB6_RS24850
• Protein ID: WP_003113526.1
• Coordinates: 5242340..5242618 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PAKB6_RS24845
• Protein ID: WP_003113527.1
• Coordinates: 5242023..5242328 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAKB6_RS24810	5237127..5237417	-	291	WP_033992920.1	DUF5447 family protein	-
PAKB6_RS24820	5237629..5237901	-	273	WP_003115921.1	hypothetical protein	-
PAKB6_RS24830	5238431..5239537	+	1107	WP_193218117.1	protein phosphatase 2C domain-containing protein	-
PAKB6_RS24835	5239540..5240589	+	1050	WP_003113529.1	serine/threonine protein kinase	-
PAKB6_RS24840	5240586..5241659	+	1074	WP_003113528.1	serine/threonine protein kinase	-
PAKB6_RS24845	5242023..5242328	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
PAKB6_RS24850	5242340..5242618	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PAKB6_RS24860	5242947..5245175	+	2229	WP_003113525.1	TonB-dependent receptor	-
PAKB6_RS24865	5245245..5245892	-	648	WP_003095021.1	carbonate dehydratase	-
PAKB6_RS24870	5245954..5247192	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T116015 WP_003113526.1 NZ_CP034429:c5242618-5242340 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T116015 NZ_CP034429:c5242618-5242340 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT116015 WP_003113527.1 NZ_CP034429:c5242328-5242023 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT116015 NZ_CP034429:c5242328-5242023 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3