Detailed information of TA system TA116001

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 127203..127456
• Replicon: plasmid pKPC-CR-hvKP-C789
• Accession: NZ_CP034417
• Organism: Klebsiella pneumoniae strain C789

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: EJJ25_RS28600
• Protein ID: WP_001312851.1
• Coordinates: 127307..127456 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	127203..127262 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJJ25_RS28555	122236..122541	+	306	WP_004144424.1	type IV conjugative transfer system protein TraL	-
EJJ25_RS28560	122561..122926	+	366	Protein_163	type IV conjugative transfer system protein TraE	-
EJJ25_RS28565	122981..123685	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
EJJ25_RS28570	123749..123910	+	162	Protein_165	DNA helicase	-
EJJ25_RS28575	123930..124676	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
EJJ25_RS28580	124731..125291	+	561	WP_000139328.1	fertility inhibition protein FinO	-
EJJ25_RS28585	125423..125623	+	201	WP_015059022.1	hypothetical protein	-
EJJ25_RS28590	126009..126608	+	600	WP_032083981.1	hypothetical protein	-
EJJ25_RS28595	126772..127002	+	231	WP_001736714.1	hypothetical protein	-
-	127203..127262	-	60	NuclAT_1	-	Antitoxin
-	127203..127262	-	60	NuclAT_1	-	Antitoxin
-	127203..127262	-	60	NuclAT_1	-	Antitoxin
-	127203..127262	-	60	NuclAT_1	-	Antitoxin
EJJ25_RS28600	127307..127456	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
EJJ25_RS28605	127740..127988	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..128299	128299
	-	flank	IS/Tn	-	-	122981..123685	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T116001 WP_001312851.1 NZ_CP034417:127307-127456 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T116001 NZ_CP034417:127307-127456 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT116001 NZ_CP034417:c127262-127203 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin