Detailed information of TA system TA115993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 60067..60794
• Replicon: plasmid pVir-CR-hvKP-C789
• Accession: NZ_CP034416
• Organism: Klebsiella pneumoniae strain C789

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: EJJ25_RS26890
• Protein ID: WP_011251285.1
• Coordinates: 60483..60794 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EJJ25_RS26885
• Protein ID: WP_011251286.1
• Coordinates: 60067..60486 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJJ25_RS26855	55235..55705	-	471	WP_048333570.1	hypothetical protein	-
EJJ25_RS30275	55898..56653	-	756	Protein_51	hypothetical protein	-
EJJ25_RS26865	57249..57692	+	444	WP_048333569.1	hypothetical protein	-
EJJ25_RS26870	57713..58501	+	789	WP_040217257.1	hypothetical protein	-
EJJ25_RS26875	58515..58850	+	336	Protein_54	conjugal transfer protein TraI	-
EJJ25_RS26880	58952..59920	-	969	WP_074428168.1	IS5 family transposase	-
EJJ25_RS26885	60067..60486	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
EJJ25_RS26890	60483..60794	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
EJJ25_RS26895	60999..61457	-	459	Protein_58	DDE-type integrase/transposase/recombinase	-
EJJ25_RS26900	61571..62268	+	698	Protein_59	IS1 family transposase	-
EJJ25_RS26905	62285..62719	+	435	Protein_60	thioredoxin fold domain-containing protein	-
EJJ25_RS26910	62736..63047	+	312	WP_011251282.1	hypothetical protein	-
EJJ25_RS26915	63061..63402	+	342	WP_011251281.1	hypothetical protein	-
EJJ25_RS26920	63462..64418	+	957	WP_011251280.1	DsbA family protein	-
EJJ25_RS26925	64843..65283	-	441	WP_011251275.1	hypothetical protein	-
EJJ25_RS26930	65273..65783	-	511	Protein_65	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..215950	215950
	-	inside	IScluster/Tn	-	-	43396..67703	24307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T115993 WP_011251285.1 NZ_CP034416:c60794-60483 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T115993 NZ_CP034416:c60794-60483 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT115993 WP_011251286.1 NZ_CP034416:c60486-60067 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT115993 NZ_CP034416:c60486-60067 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1