Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4048458..4049053 Replicon chromosome
Accession NZ_CP034409
Organism Pseudomonas aeruginosa strain SP4527

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag EJA97_RS19180 Protein ID WP_003113526.1
Coordinates 4048775..4049053 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag EJA97_RS19175 Protein ID WP_003111575.1
Coordinates 4048458..4048763 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJA97_RS19140 4043598..4044446 + 849 WP_031686602.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
EJA97_RS19150 4044613..4045554 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
EJA97_RS19155 4045671..4046285 + 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
EJA97_RS19160 4046327..4046911 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
EJA97_RS19165 4046952..4048052 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
EJA97_RS19175 4048458..4048763 - 306 WP_003111575.1 HigA family addiction module antidote protein Antitoxin
EJA97_RS19180 4048775..4049053 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EJA97_RS19190 4049382..4051610 + 2229 WP_033991161.1 TonB-dependent receptor -
EJA97_RS19195 4051681..4052328 - 648 WP_003095021.1 carbonate dehydratase -
EJA97_RS19200 4052390..4053628 - 1239 WP_031686604.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T115969 WP_003113526.1 NZ_CP034409:c4049053-4048775 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T115969 NZ_CP034409:c4049053-4048775 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGACGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT115969 WP_003111575.1 NZ_CP034409:c4048763-4048458 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT115969 NZ_CP034409:c4048763-4048458 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References