Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4940224..4940821 Replicon chromosome
Accession NZ_CP034408
Organism Klebsiella pneumoniae strain NH34

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag EJC76_RS26880 Protein ID WP_004142563.1
Coordinates 4940224..4940541 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag EJC76_RS26885 Protein ID WP_004142561.1
Coordinates 4940534..4940821 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC76_RS26860 4935926..4937353 - 1428 WP_004176980.1 MFS transporter -
EJC76_RS26865 4937462..4938329 + 868 Protein_4684 helix-turn-helix domain-containing protein -
EJC76_RS26875 4938996..4940039 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
EJC76_RS26880 4940224..4940541 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EJC76_RS26885 4940534..4940821 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
EJC76_RS26890 4941089..4941739 - 651 Protein_4688 oxygen-insensitive NAD(P)H nitroreductase -
EJC76_RS26895 4941859..4942227 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
EJC76_RS26900 4942227..4942808 - 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
EJC76_RS26905 4942988..4944103 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
EJC76_RS26910 4944134..4944475 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
EJC76_RS26915 4944493..4944741 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T115963 WP_004142563.1 NZ_CP034408:4940224-4940541 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T115963 NZ_CP034408:4940224-4940541 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT115963 WP_004142561.1 NZ_CP034408:4940534-4940821 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT115963 NZ_CP034408:4940534-4940821 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References