Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4394951..4395492 Replicon chromosome
Accession NZ_CP034404
Organism Escherichia coli strain CRE10

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EJC75_RS23155 Protein ID WP_000615976.1
Coordinates 4394951..4395229 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EJC75_RS23160 Protein ID WP_000729704.1
Coordinates 4395232..4395492 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC75_RS23140 4392533..4393111 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EJC75_RS23145 4393317..4394084 + 768 WP_000333380.1 class II glutamine amidotransferase -
EJC75_RS23150 4394055..4394795 - 741 WP_001225679.1 murein L,D-transpeptidase -
EJC75_RS23155 4394951..4395229 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EJC75_RS23160 4395232..4395492 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EJC75_RS23165 4395678..4396451 + 774 WP_000543895.1 C40 family peptidase -
EJC75_RS23170 4396508..4396864 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
EJC75_RS23175 4396857..4397135 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EJC75_RS23180 4397240..4398952 - 1713 Protein_4080 flagellar biosynthesis protein FlhA -
EJC75_RS23185 4398924..4399709 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T115940 WP_000615976.1 NZ_CP034404:c4395229-4394951 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T115940 NZ_CP034404:c4395229-4394951 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT115940 WP_000729704.1 NZ_CP034404:c4395492-4395232 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT115940 NZ_CP034404:c4395492-4395232 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References