Detailed information of TA system TA115936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3531762..3532364
• Replicon: chromosome
• Accession: NZ_CP034404
• Organism: Escherichia coli strain CRE10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EJC75_RS19115
• Protein ID: WP_000897305.1
• Coordinates: 3531762..3532073 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EJC75_RS19120
• Protein ID: WP_000356397.1
• Coordinates: 3532074..3532364 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC75_RS19085	3526792..3527577	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
EJC75_RS19090	3527676..3528275	+	600	WP_001295269.1	glucose-1-phosphatase	-
EJC75_RS19095	3528269..3529141	+	873	WP_000920762.1	virulence factor BrkB family protein	-
EJC75_RS19100	3529138..3529575	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EJC75_RS19105	3529620..3530561	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EJC75_RS19110	3530625..3531533	-	909	WP_001162704.1	alpha/beta hydrolase	-
EJC75_RS19115	3531762..3532073	+	312	WP_000897305.1	hypothetical protein	Toxin
EJC75_RS19120	3532074..3532364	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EJC75_RS19125	3532969..3533187	+	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
EJC75_RS19130	3533406..3533648	+	243	WP_001086388.1	hypothetical protein	-
EJC75_RS19135	3533978..3534907	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EJC75_RS19140	3534904..3535539	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EJC75_RS19145	3535536..3536438	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T115936 WP_000897305.1 NZ_CP034404:3531762-3532073 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T115936 NZ_CP034404:3531762-3532073 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT115936 WP_000356397.1 NZ_CP034404:3532074-3532364 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT115936 NZ_CP034404:3532074-3532364 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7