115930

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1488017..1488519 Replicon chromosome
Accession NZ_CP034404
Organism Escherichia coli strain CRE10

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EJC75_RS09245 Protein ID WP_000767829.1
Coordinates 1488017..1488271 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EJC75_RS09250 Protein ID WP_001259255.1
Coordinates 1488268..1488519 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC75_RS09215 1483032..1483259 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EJC75_RS09220 1483273..1484331 - 1059 WP_000492346.1 transport protein YeeE -
EJC75_RS09225 1484510..1485868 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EJC75_RS09230 1485858..1485920 - 63 WP_010723108.1 membrane protein YoeI -
EJC75_RS09235 1486135..1487064 - 930 WP_000803351.1 LysR family transcriptional regulator -
EJC75_RS09240 1487110..1487934 - 825 WP_000754758.1 SDR family oxidoreductase -
EJC75_RS09245 1488017..1488271 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EJC75_RS09250 1488268..1488519 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EJC75_RS09255 1488802..1488852 + 51 WP_001364200.1 his operon leader peptide -
EJC75_RS09260 1488998..1489897 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EJC75_RS09265 1489903..1491207 + 1305 WP_001542955.1 histidinol dehydrogenase -
EJC75_RS09270 1491204..1492274 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
EJC75_RS09275 1492274..1493341 + 1068 WP_000080130.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T115930 WP_000767829.1 NZ_CP034404:c1488271-1488017 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T115930 NZ_CP034404:c1488271-1488017 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT115930 WP_001259255.1 NZ_CP034404:c1488519-1488268 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT115930 NZ_CP034404:c1488519-1488268 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References