Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1488017..1488519 Replicon chromosome
Accession NZ_CP034404
Organism Escherichia coli strain CRE10

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EJC75_RS09245 Protein ID WP_000767829.1
Coordinates 1488017..1488271 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EJC75_RS09250 Protein ID WP_001259255.1
Coordinates 1488268..1488519 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC75_RS09215 1483032..1483259 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EJC75_RS09220 1483273..1484331 - 1059 WP_000492346.1 transport protein YeeE -
EJC75_RS09225 1484510..1485868 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EJC75_RS09230 1485858..1485920 - 63 WP_010723108.1 membrane protein YoeI -
EJC75_RS09235 1486135..1487064 - 930 WP_000803351.1 LysR family transcriptional regulator -
EJC75_RS09240 1487110..1487934 - 825 WP_000754758.1 SDR family oxidoreductase -
EJC75_RS09245 1488017..1488271 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EJC75_RS09250 1488268..1488519 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EJC75_RS09255 1488802..1488852 + 51 WP_001364200.1 his operon leader peptide -
EJC75_RS09260 1488998..1489897 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EJC75_RS09265 1489903..1491207 + 1305 WP_001542955.1 histidinol dehydrogenase -
EJC75_RS09270 1491204..1492274 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
EJC75_RS09275 1492274..1493341 + 1068 WP_000080130.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T115930 WP_000767829.1 NZ_CP034404:c1488271-1488017 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T115930 NZ_CP034404:c1488271-1488017 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT115930 WP_001259255.1 NZ_CP034404:c1488519-1488268 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT115930 NZ_CP034404:c1488519-1488268 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References