Detailed information of TA system TA115918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 26628..27285
• Replicon: plasmid pCRE10.2
• Accession: NZ_CP034402
• Organism: Escherichia coli strain CRE10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: EJC75_RS01125
• Protein ID: WP_000270043.1
• Coordinates: 26628..26978 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EJC75_RS01130
• Protein ID: WP_000124640.1
• Coordinates: 26983..27285 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC75_RS01085	23102..23599	-	498	WP_000062185.1	hypothetical protein	-
EJC75_RS01090	23602..24090	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
EJC75_RS01095	24187..24522	+	336	WP_000683477.1	hypothetical protein	-
EJC75_RS01100	24537..25007	-	471	WP_001281821.1	hypothetical protein	-
EJC75_RS01105	25000..25371	-	372	WP_000516918.1	hypothetical protein	-
EJC75_RS01110	25382..25576	-	195	WP_000343597.1	hypothetical protein	-
EJC75_RS01120	25917..26465	-	549	WP_001061573.1	hypothetical protein	-
EJC75_RS01125	26628..26978	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJC75_RS01130	26983..27285	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
EJC75_RS01135	27312..27605	-	294	WP_001239998.1	hypothetical protein	-
EJC75_RS01140	27694..27966	-	273	WP_001043046.1	HU family DNA-binding protein	-
EJC75_RS01145	28024..28551	-	528	WP_001210659.1	thermonuclease family protein	-
EJC75_RS26390	28554..28859	-	306	WP_171820680.1	hypothetical protein	-
EJC75_RS01155	28771..29628	-	858	WP_001167036.1	hypothetical protein	-
EJC75_RS01160	29615..29845	-	231	WP_000972665.1	hypothetical protein	-
EJC75_RS01165	29845..30363	-	519	WP_000210757.1	nitrite reductase	-
EJC75_RS01170	30360..30806	-	447	WP_000919343.1	hypothetical protein	-
EJC75_RS01175	30806..31165	-	360	WP_000422769.1	hypothetical protein	-
EJC75_RS01180	31223..31651	-	429	WP_000591076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	galE	1..92446	92446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T115918 WP_000270043.1 NZ_CP034402:26628-26978 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T115918 NZ_CP034402:26628-26978 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT115918 WP_000124640.1 NZ_CP034402:26983-27285 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT115918 NZ_CP034402:26983-27285 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9