Detailed information of TA system TA115914

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 106989..107228
• Replicon: plasmid pCRE10.1
• Accession: NZ_CP034401
• Organism: Escherichia coli strain CRE10

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: EJC75_RS00760
• Protein ID: WP_023144756.1
• Coordinates: 106989..107123 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	107168..107228 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC75_RS00730	102706..103563	-	858	WP_000130646.1	incFII family plasmid replication initiator RepA	-
EJC75_RS00735	103556..103630	-	75	WP_014966221.1	RepA leader peptide Tap	-
EJC75_RS26365	103627..103761	-	135	Protein_126	protein CopA/IncA	-
EJC75_RS00745	103992..105533	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
EJC75_RS00750	105548..106278	+	731	Protein_128	IS21-like element ISEc12 family helper ATPase IstB	-
EJC75_RS00755	106438..106692	-	255	WP_000083850.1	replication regulatory protein RepA	-
EJC75_RS00760	106989..107123	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	107168..107228	+	61	NuclAT_2	-	Antitoxin
-	107168..107228	+	61	NuclAT_2	-	Antitoxin
-	107168..107228	+	61	NuclAT_2	-	Antitoxin
-	107168..107228	+	61	NuclAT_2	-	Antitoxin
EJC75_RS26120	107195..107481	-	287	Protein_131	DUF2726 domain-containing protein	-
EJC75_RS00775	107994..108206	-	213	WP_013023861.1	hypothetical protein	-
EJC75_RS00780	108337..108897	-	561	WP_000139341.1	fertility inhibition protein FinO	-
EJC75_RS00785	108952..109698	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / catB3 / blaOXA-1 / aac(6')-Ib-cr / dfrA17 / aadA5 / qacE / mph(A) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId	-	1..134611	134611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T115914 WP_023144756.1 NZ_CP034401:c107123-106989 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T115914 NZ_CP034401:c107123-106989 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT115914 NZ_CP034401:107168-107228 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin