Detailed information of TA system TA115907

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 15486..15726
• Replicon: plasmid pCRE10.1
• Accession: NZ_CP034401
• Organism: Escherichia coli strain CRE10

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EJC75_RS00120
• Protein ID: WP_001312861.1
• Coordinates: 15486..15644 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	15688..15726 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC75_RS00085	11061..11663	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
EJC75_RS00090	11960..12781	-	822	WP_001234469.1	DUF945 domain-containing protein	-
EJC75_RS00095	12900..13187	-	288	WP_000107535.1	hypothetical protein	-
EJC75_RS00100	13438..14418	+	981	WP_032154316.1	IS110 family transposase	-
EJC75_RS26355	14604..14795	-	192	Protein_20	single-stranded DNA-binding protein	-
EJC75_RS00120	15486..15644	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	15688..15726	-	39	NuclAT_1	-	Antitoxin
-	15688..15726	-	39	NuclAT_1	-	Antitoxin
-	15688..15726	-	39	NuclAT_1	-	Antitoxin
-	15688..15726	-	39	NuclAT_1	-	Antitoxin
-	17166..17268	-	103	NuclAT_0	-	-
-	17166..17268	-	103	NuclAT_0	-	-
-	17166..17268	-	103	NuclAT_0	-	-
-	17166..17268	-	103	NuclAT_0	-	-
EJC75_RS00130	17280..17999	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
EJC75_RS00135	17996..18430	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
EJC75_RS00140	18485..20443	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / catB3 / blaOXA-1 / aac(6')-Ib-cr / dfrA17 / aadA5 / qacE / mph(A) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId	-	1..134611	134611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T115907 WP_001312861.1 NZ_CP034401:c15644-15486 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T115907 NZ_CP034401:c15644-15486 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGAGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 39 bp

>AT115907 NZ_CP034401:c15726-15688 [Escherichia coli]
CATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin