Detailed information of TA system TA115895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2409613..2410215
• Replicon: chromosome
• Accession: NZ_CP034399
• Organism: Escherichia coli strain CRE1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EJC48_RS13570
• Protein ID: WP_000897305.1
• Coordinates: 2409613..2409924 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EJC48_RS13575
• Protein ID: WP_000356397.1
• Coordinates: 2409925..2410215 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC48_RS13540	2404645..2405430	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
EJC48_RS13545	2405529..2406126	+	598	Protein_2235	glucose-1-phosphatase	-
EJC48_RS13550	2406120..2406992	+	873	WP_000920762.1	virulence factor BrkB family protein	-
EJC48_RS13555	2406989..2407426	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EJC48_RS13560	2407471..2408412	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EJC48_RS13565	2408476..2409384	-	909	WP_001162704.1	alpha/beta hydrolase	-
EJC48_RS13570	2409613..2409924	+	312	WP_000897305.1	hypothetical protein	Toxin
EJC48_RS13575	2409925..2410215	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EJC48_RS13580	2410820..2411038	+	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
EJC48_RS13585	2411257..2411499	+	243	WP_001086388.1	hypothetical protein	-
EJC48_RS13590	2411829..2412758	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EJC48_RS13595	2412755..2413390	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EJC48_RS13600	2413387..2414289	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T115895 WP_000897305.1 NZ_CP034399:2409613-2409924 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T115895 NZ_CP034399:2409613-2409924 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT115895 WP_000356397.1 NZ_CP034399:2409925-2410215 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT115895 NZ_CP034399:2409925-2410215 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7