Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 367249..367751 Replicon chromosome
Accession NZ_CP034399
Organism Escherichia coli strain CRE1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EJC48_RS03680 Protein ID WP_000767829.1
Coordinates 367249..367503 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EJC48_RS03685 Protein ID WP_001259255.1
Coordinates 367500..367751 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJC48_RS03650 362264..362491 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EJC48_RS03655 362505..363563 - 1059 WP_000492346.1 transport protein YeeE -
EJC48_RS03660 363742..365100 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EJC48_RS03665 365090..365152 - 63 WP_010723108.1 membrane protein YoeI -
EJC48_RS03670 365367..366296 - 930 WP_000803351.1 LysR family transcriptional regulator -
EJC48_RS03675 366342..367166 - 825 WP_000754758.1 SDR family oxidoreductase -
EJC48_RS03680 367249..367503 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EJC48_RS03685 367500..367751 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EJC48_RS03690 368034..368084 + 51 WP_001364200.1 his operon leader peptide -
EJC48_RS03695 368230..369129 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EJC48_RS03700 369135..370439 + 1305 WP_001542955.1 histidinol dehydrogenase -
EJC48_RS03705 370436..371506 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
EJC48_RS03710 371506..372573 + 1068 WP_000080130.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T115889 WP_000767829.1 NZ_CP034399:c367503-367249 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T115889 NZ_CP034399:c367503-367249 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT115889 WP_001259255.1 NZ_CP034399:c367751-367500 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT115889 NZ_CP034399:c367751-367500 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References