Detailed information of TA system TA115879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 62578..63235
• Replicon: plasmid pCRE1.2
• Accession: NZ_CP034397
• Organism: Escherichia coli strain CRE1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: EJC48_RS01275
• Protein ID: WP_000270043.1
• Coordinates: 62885..63235 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EJC48_RS01270
• Protein ID: WP_000124640.1
• Coordinates: 62578..62880 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC48_RS01220	58162..58590	+	429	WP_000591076.1	hypothetical protein	-
EJC48_RS01225	58648..59007	+	360	WP_000422769.1	hypothetical protein	-
EJC48_RS01230	59096..59503	+	408	WP_129498915.1	Fe3+-siderophore ABC transporter permease	-
EJC48_RS01235	59500..60018	+	519	WP_000210757.1	nitrite reductase	-
EJC48_RS01240	60018..60248	+	231	WP_000972665.1	hypothetical protein	-
EJC48_RS01245	60235..61092	+	858	WP_001167036.1	hypothetical protein	-
EJC48_RS26390	61004..61309	+	306	WP_171820680.1	hypothetical protein	-
EJC48_RS01255	61312..61839	+	528	WP_001210659.1	thermonuclease family protein	-
EJC48_RS01260	61897..62169	+	273	WP_001043046.1	HU family DNA-binding protein	-
EJC48_RS01265	62258..62551	+	294	WP_001239998.1	hypothetical protein	-
EJC48_RS01270	62578..62880	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
EJC48_RS01275	62885..63235	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJC48_RS01280	63398..63946	+	549	WP_001061573.1	hypothetical protein	-
EJC48_RS01290	64287..64481	+	195	WP_000343597.1	hypothetical protein	-
EJC48_RS01295	64492..64863	+	372	WP_000516918.1	hypothetical protein	-
EJC48_RS01300	64856..65326	+	471	WP_001281821.1	hypothetical protein	-
EJC48_RS01305	65341..65676	-	336	WP_000683477.1	hypothetical protein	-
EJC48_RS01310	65773..66261	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
EJC48_RS01315	66264..66761	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..93642	93642

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T115879 WP_000270043.1 NZ_CP034397:c63235-62885 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T115879 NZ_CP034397:c63235-62885 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT115879 WP_000124640.1 NZ_CP034397:c62880-62578 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT115879 NZ_CP034397:c62880-62578 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9