Detailed information of TA system TA115872

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69823..70063
• Replicon: plasmid pCRE1.1
• Accession: NZ_CP034396
• Organism: Escherichia coli strain CRE1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EJC48_RS00460
• Protein ID: WP_001312861.1
• Coordinates: 69823..69981 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	70025..70063 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJC48_RS00425	65398..66000	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
EJC48_RS00430	66297..67118	-	822	WP_001234469.1	DUF945 domain-containing protein	-
EJC48_RS00435	67237..67524	-	288	WP_000107535.1	hypothetical protein	-
EJC48_RS00440	67775..68755	+	981	WP_032154316.1	IS110 family transposase	-
EJC48_RS26385	68941..69132	-	192	Protein_83	single-stranded DNA-binding protein	-
EJC48_RS00460	69823..69981	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	70025..70063	-	39	NuclAT_1	-	Antitoxin
-	70025..70063	-	39	NuclAT_1	-	Antitoxin
-	70025..70063	-	39	NuclAT_1	-	Antitoxin
-	70025..70063	-	39	NuclAT_1	-	Antitoxin
-	71503..71605	-	103	NuclAT_0	-	-
-	71503..71605	-	103	NuclAT_0	-	-
-	71503..71605	-	103	NuclAT_0	-	-
-	71503..71605	-	103	NuclAT_0	-	-
EJC48_RS00470	71617..72336	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
EJC48_RS00475	72333..72767	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
EJC48_RS00480	72822..74780	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId / tet(B) / catB3 / blaOXA-1 / aac(6')-Ib-cr / dfrA17 / aadA5	-	1..134635	134635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T115872 WP_001312861.1 NZ_CP034396:c69981-69823 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T115872 NZ_CP034396:c69981-69823 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGAGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 39 bp

>AT115872 NZ_CP034396:c70063-70025 [Escherichia coli]
CATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin