Detailed information of TA system TA115859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4866664..4867266
• Replicon: chromosome
• Accession: NZ_CP034389
• Organism: Escherichia coli strain RS571

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EJH97_RS23895
• Protein ID: WP_000897305.1
• Coordinates: 4866955..4867266 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EJH97_RS23890
• Protein ID: WP_000356397.1
• Coordinates: 4866664..4866954 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJH97_RS23865	4863166..4864068	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
EJH97_RS23870	4864065..4864700	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EJH97_RS23875	4864697..4865626	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
EJH97_RS23880	4865841..4866059	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
EJH97_RS23890	4866664..4866954	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EJH97_RS23895	4866955..4867266	-	312	WP_000897305.1	hypothetical protein	Toxin
EJH97_RS23900	4867495..4868403	+	909	WP_001318161.1	alpha/beta hydrolase	-
EJH97_RS23905	4868467..4869408	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EJH97_RS23910	4869453..4869890	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EJH97_RS23915	4869887..4870759	-	873	WP_000920762.1	virulence factor BrkB family protein	-
EJH97_RS23920	4870753..4871352	-	600	WP_001314324.1	glucose-1-phosphatase	-
EJH97_RS23925	4871451..4872236	-	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T115859 WP_000897305.1 NZ_CP034389:c4867266-4866955 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T115859 NZ_CP034389:c4867266-4866955 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT115859 WP_000356397.1 NZ_CP034389:c4866954-4866664 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT115859 NZ_CP034389:c4866954-4866664 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7