Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3913042..3913736 Replicon chromosome
Accession NZ_CP034389
Organism Escherichia coli strain RS571

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag EJH97_RS19210 Protein ID WP_001263491.1
Coordinates 3913042..3913440 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag EJH97_RS19215 Protein ID WP_000554755.1
Coordinates 3913443..3913736 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJH97_RS19180 3908304..3909761 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
EJH97_RS19185 3909770..3910051 + 282 WP_022645226.1 hypothetical protein -
EJH97_RS19190 3910068..3910577 - 510 WP_001361775.1 hydrolase -
EJH97_RS19195 3910639..3911253 - 615 WP_022645225.1 peptide chain release factor H -
EJH97_RS19200 3911250..3912389 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
EJH97_RS19205 3912580..3913032 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
EJH97_RS19210 3913042..3913440 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EJH97_RS19215 3913443..3913736 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EJH97_RS19220 3913788..3914843 - 1056 WP_022645222.1 DNA polymerase IV -
EJH97_RS19225 3914914..3915699 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
EJH97_RS19230 3915671..3917383 + 1713 Protein_3708 flagellar biosynthesis protein FlhA -
EJH97_RS19235 3917481..3918254 - 774 WP_022645219.1 C40 family peptidase -
EJH97_RS19240 3918440..3918700 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T115852 WP_001263491.1 NZ_CP034389:c3913440-3913042 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T115852 NZ_CP034389:c3913440-3913042 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT115852 WP_000554755.1 NZ_CP034389:c3913736-3913443 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT115852 NZ_CP034389:c3913736-3913443 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7


Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References