Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1974096..1974598 Replicon chromosome
Accession NZ_CP034389
Organism Escherichia coli strain RS571

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EJH97_RS09530 Protein ID WP_000767829.1
Coordinates 1974344..1974598 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EJH97_RS09525 Protein ID WP_001259255.1
Coordinates 1974096..1974347 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJH97_RS09500 1969274..1970341 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EJH97_RS09505 1970341..1971411 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
EJH97_RS09510 1971408..1972712 - 1305 WP_021523144.1 histidinol dehydrogenase -
EJH97_RS09515 1972718..1973617 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
EJH97_RS09520 1973763..1973813 - 51 WP_001364200.1 his operon leader peptide -
EJH97_RS09525 1974096..1974347 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EJH97_RS09530 1974344..1974598 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EJH97_RS09535 1974681..1975505 + 825 WP_000754758.1 SDR family oxidoreductase -
EJH97_RS09540 1975551..1976480 + 930 WP_023154044.1 LysR family transcriptional regulator -
EJH97_RS09545 1976695..1976757 + 63 WP_010723108.1 membrane protein YoeI -
EJH97_RS09550 1976747..1978105 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EJH97_RS09555 1978284..1979342 + 1059 WP_000492324.1 transport protein YeeE -
EJH97_RS09560 1979356..1979583 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T115838 WP_000767829.1 NZ_CP034389:1974344-1974598 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T115838 NZ_CP034389:1974344-1974598 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT115838 WP_001259255.1 NZ_CP034389:1974096-1974347 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT115838 NZ_CP034389:1974096-1974347 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References