Detailed information of TA system TA115781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 63800..64445
• Replicon: chromosome
• Accession: NZ_CP034363
• Organism: Pantoea sp. CCBC3-3-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EHV07_RS00265
• Protein ID: WP_147193725.1
• Coordinates: 64083..64445 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EHV07_RS00260
• Protein ID: WP_147193723.1
• Coordinates: 63800..64099 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHV07_RS00240	59029..59319	+	291	WP_147193715.1	HTH-type transcriptional regulator	-
EHV07_RS00245	59437..60489	+	1053	WP_147193717.1	NAD(P)-dependent alcohol dehydrogenase	-
EHV07_RS00250	60647..62716	-	2070	WP_147193719.1	glycine--tRNA ligase subunit beta	-
EHV07_RS00255	62726..63634	-	909	WP_147193721.1	glycine--tRNA ligase subunit alpha	-
EHV07_RS00260	63800..64099	-	300	WP_147193723.1	XRE family transcriptional regulator	Antitoxin
EHV07_RS00265	64083..64445	-	363	WP_147193725.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EHV07_RS00270	64584..65579	+	996	WP_147193727.1	acyltransferase	-
EHV07_RS00275	65602..68334	-	2733	WP_147193729.1	HTH-type transcriptional regulator MalT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-84)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14139.21 Da
• Isoelectric Point: 8.0157

>T115781 WP_147193725.1 NZ_CP034363:c64445-64083 [Pantoea sp. CCBC3-3-1]
MWEIITTARFDRWFHEQSEDLQDEILSAFIVLRKEGPMTGRPLVDTLNDSKYANMKELRLSFEARPIRALFAFDPVRQAI
FLCAGDKTGDRKFYKKYIKIADAEYKAHLARLEFDNGNFR

Nucleotide

• Length: 363 bp

>T115781 NZ_CP034363:c64445-64083 [Pantoea sp. CCBC3-3-1]
ATGTGGGAAATCATCACGACCGCGCGATTTGACAGGTGGTTCCATGAACAATCAGAGGATTTGCAAGACGAGATTTTATC
TGCATTTATCGTATTGCGAAAAGAAGGTCCGATGACTGGACGGCCACTGGTAGACACTTTAAACGACTCTAAATATGCAA
ATATGAAGGAGTTACGCCTTTCATTTGAGGCCAGACCGATCAGAGCGCTGTTCGCATTTGACCCGGTCCGCCAGGCTATT
TTCCTTTGTGCTGGCGACAAGACAGGCGACCGAAAATTTTATAAAAAATACATCAAAATAGCCGATGCTGAATACAAAGC
TCATCTGGCAAGACTGGAGTTTGATAATGGCAACTTTAGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11070.97 Da
• Isoelectric Point: 8.5308

>AT115781 WP_147193723.1 NZ_CP034363:c64099-63800 [Pantoea sp. CCBC3-3-1]
MATLDELLKEVSPERRAKIEVRAEEMIREITLAQMREELQISQTALAIALGVKQPAVARMERVDNDPRLSTLKRYVAALG
GELSIDIKLPSGKRVAYPL

Nucleotide

• Length: 300 bp

>AT115781 NZ_CP034363:c64099-63800 [Pantoea sp. CCBC3-3-1]
ATGGCAACTTTAGATGAACTACTGAAAGAGGTAAGTCCAGAACGCCGGGCCAAAATCGAGGTTCGCGCCGAAGAAATGAT
CCGCGAAATCACTCTCGCGCAAATGCGTGAAGAGCTGCAAATTTCACAAACTGCACTCGCCATAGCGCTGGGCGTTAAAC
AACCTGCCGTAGCCCGTATGGAACGTGTTGATAATGACCCGCGCCTGTCCACTCTCAAACGCTATGTAGCCGCGCTGGGC
GGGGAACTTTCTATCGACATAAAACTTCCATCCGGTAAACGAGTGGCTTACCCACTGTAA