Detailed information of TA system TA115775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1959596..1960191
• Replicon: chromosome
• Accession: NZ_CP034354
• Organism: Pseudomonas aeruginosa strain IMP-13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: EIP87_RS09480
• Protein ID: WP_003113526.1
• Coordinates: 1959913..1960191 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EIP87_RS09475
• Protein ID: WP_043096613.1
• Coordinates: 1959596..1959901 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EIP87_RS09440	1954736..1955584	+	849	WP_003117426.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
EIP87_RS09450	1955751..1956692	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
EIP87_RS09455	1956809..1957423	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
EIP87_RS09460	1957465..1958049	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
EIP87_RS09465	1958090..1959190	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
EIP87_RS09475	1959596..1959901	-	306	WP_043096613.1	HigA family addiction module antidote protein	Antitoxin
EIP87_RS09480	1959913..1960191	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EIP87_RS09490	1960520..1962748	+	2229	WP_043096614.1	TonB-dependent receptor	-
EIP87_RS09495	1962819..1963466	-	648	WP_003095021.1	carbonate dehydratase	-
EIP87_RS09500	1963528..1964766	-	1239	WP_023084819.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T115775 WP_003113526.1 NZ_CP034354:c1960191-1959913 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T115775 NZ_CP034354:c1960191-1959913 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11239.74 Da
• Isoelectric Point: 4.4475

>AT115775 WP_043096613.1 NZ_CP034354:c1959901-1959596 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT115775 NZ_CP034354:c1959901-1959596 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3