Detailed information of TA system TA115701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 46718..47334
• Replicon: plasmid pVPSD2016-3
• Accession: NZ_CP034302
• Organism: Vibrio parahaemolyticus strain 20160303005-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EHC69_RS28265
• Protein ID: WP_086482456.1
• Coordinates: 47047..47334 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EHC69_RS28260
• Protein ID: WP_086482455.1
• Coordinates: 46718..47035 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHC69_RS28235	42245..43186	+	942	WP_086482451.1	TIGR03756 family integrating conjugative element protein	-
EHC69_RS28240	43252..44499	+	1248	WP_170927767.1	integrating conjugative element protein	-
EHC69_RS28245	44509..44784	+	276	WP_086482452.1	hypothetical protein	-
EHC69_RS28250	44775..46355	+	1581	WP_086482453.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
EHC69_RS28255	46384..46629	-	246	WP_086482454.1	hypothetical protein	-
EHC69_RS28260	46718..47035	-	318	WP_086482455.1	HigA family addiction module antidote protein	Antitoxin
EHC69_RS28265	47047..47334	-	288	WP_086482456.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EHC69_RS28270	47501..48052	-	552	WP_086482457.1	DUF5384 family protein	-
EHC69_RS28275	48058..48564	-	507	WP_086482458.1	hypothetical protein	-
EHC69_RS28280	48722..49315	+	594	WP_086482459.1	hypothetical protein	-
EHC69_RS28285	49591..50934	+	1344	WP_086482460.1	outer membrane protein transport protein	-
EHC69_RS28290	51271..51462	+	192	WP_086482461.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96431	96431

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11272.91 Da
• Isoelectric Point: 7.3567

>T115701 WP_086482456.1 NZ_CP034302:c47334-47047 [Vibrio parahaemolyticus]
MAVNFKAEWLEAFYEEDKHHRLIPKTIENALFRKLEILDAAKAESDLRVPPGNRFEHLEGNLKGWCSIRVNKQYRLIFKW
VNGVAEDTYLDPHKY

Nucleotide

• Length: 288 bp

>T115701 NZ_CP034302:c47334-47047 [Vibrio parahaemolyticus]
ATGGCAGTAAATTTTAAAGCGGAATGGCTTGAAGCATTTTACGAAGAGGACAAGCACCATCGGTTGATCCCTAAAACTAT
TGAAAATGCATTGTTTCGCAAACTTGAAATCTTAGATGCCGCAAAAGCAGAGTCAGACTTAAGGGTTCCCCCAGGCAATC
GGTTTGAACATCTTGAAGGTAATCTGAAGGGGTGGTGCTCAATACGAGTAAATAAACAATATCGTTTGATTTTTAAGTGG
GTTAACGGTGTGGCAGAAGACACCTATTTAGACCCTCATAAATACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11931.86 Da
• Isoelectric Point: 10.2653

>AT115701 WP_086482455.1 NZ_CP034302:c47035-46718 [Vibrio parahaemolyticus]
MRRTKRRPVSVGEMLKLEFLEPMEITSKTLAEAMGVHRNTVSNLINGGTLTAPLAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKLVKPLFSTRDIELRT

Nucleotide

• Length: 318 bp

>AT115701 NZ_CP034302:c47035-46718 [Vibrio parahaemolyticus]
ATGCGTAGAACTAAACGTCGTCCAGTCAGTGTAGGTGAAATGCTTAAGCTTGAGTTTTTAGAACCGATGGAAATCACATC
GAAAACTTTAGCAGAAGCGATGGGAGTACATAGAAATACCGTCAGTAATTTGATTAATGGTGGCACTTTAACTGCACCAC
TTGCAATTAAGTTAGCGGCAGCTTTGGGGAATACGCCGGAGTTTTGGCTTAATATTCAGCACGCTGTGGATTTGTGGGAC
ACCAGAAATCGATATCAAGAAGAAGCTAAATTAGTCAAACCTTTATTTTCAACGCGAGACATTGAATTACGTACATAA