Detailed information of TA system TA115653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3801601..3802295
• Replicon: chromosome
• Accession: NZ_CP034276
• Organism: Pectobacterium versatile strain 14A

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: EIP93_RS17245
• Protein ID: WP_206428399.1
• Coordinates: 3801601..3801999 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: EIP93_RS17250
• Protein ID: WP_027711146.1
• Coordinates: 3802002..3802295 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EIP93_RS17215	3797668..3797982	-	315	WP_039536088.1	PTS cellobiose transporter subunit IIB	-
EIP93_RS17220	3798313..3799563	-	1251	WP_125233023.1	VWA domain-containing protein	-
EIP93_RS17225	3799730..3799843	-	114	WP_107333336.1	IS3 family transposase	-
EIP93_RS17230	3800253..3800744	+	492	WP_116586571.1	helix-turn-helix domain-containing protein	-
EIP93_RS17235	3800737..3801321	+	585	WP_125233024.1	putative adenosine monophosphate-protein transferase Fic	-
EIP93_RS17245	3801601..3801999	-	399	WP_206428399.1	type II toxin-antitoxin system YafO family toxin	Toxin
EIP93_RS17250	3802002..3802295	-	294	WP_027711146.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EIP93_RS17255	3802535..3803635	+	1101	WP_125233026.1	hypothetical protein	-
EIP93_RS17260	3803960..3804520	-	561	WP_125233027.1	type IV pilin	-
EIP93_RS22825	3804676..3805899	-	1224	WP_206428381.1	hypothetical protein	-
EIP93_RS22830	3805911..3806201	-	291	WP_206428382.1	prepilin-type cleavage/methylation domain-containing protein	-
EIP93_RS17270	3806392..3806676	-	285	WP_125233028.1	conjugal transfer protein TraD	-
EIP93_RS17275	3806676..3806930	-	255	WP_125233029.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15251.54 Da
• Isoelectric Point: 7.1284

>T115653 WP_206428399.1 NZ_CP034276:c3801999-3801601 [Pectobacterium versatile]
IRIFKSALIRQQLSQQELDDLVADFLSYKKDGVLPDTFGRDAPYDDDRTYPLVKEEQVAHIHLADADAPFPKFLRQFKRT
SDQAHLVYCQGAMDPDAYLLIIILKPEAHKMARNNSHMHKIGMIAEAFRMKH

Nucleotide

• Length: 399 bp

>T115653 NZ_CP034276:c3801999-3801601 [Pectobacterium versatile]
ATCAGGATTTTCAAGTCAGCACTGATTCGCCAGCAATTAAGCCAGCAAGAGCTTGATGATTTGGTTGCGGATTTTCTGTC
CTATAAAAAAGATGGCGTGTTGCCAGATACCTTTGGTCGTGATGCACCCTATGATGATGACCGCACCTATCCCTTGGTAA
AAGAGGAGCAGGTGGCACACATTCATCTTGCCGATGCGGATGCCCCTTTCCCTAAGTTCTTGCGTCAGTTCAAACGAACC
AGCGATCAGGCGCATTTGGTATACTGCCAAGGTGCGATGGATCCCGATGCCTATCTGTTGATTATCATCCTTAAACCCGA
AGCGCATAAAATGGCACGCAACAATAGCCATATGCACAAGATCGGGATGATAGCCGAAGCGTTCAGGATGAAACATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10979.44 Da
• Isoelectric Point: 4.9167

>AT115653 WP_027711146.1 NZ_CP034276:c3802295-3802002 [Pectobacterium versatile]
MERILAEKSINITELRKNPAKYFIDEPVAVLSNNRPAGYMVSAKVFEELIDLLEEKQGRVHTAARFRPSAERLSDIADSG
QKLLQNASDKDLTEFTE

Nucleotide

• Length: 294 bp

>AT115653 NZ_CP034276:c3802295-3802002 [Pectobacterium versatile]
ATGGAACGCATCCTCGCAGAAAAATCTATCAACATCACCGAGTTAAGAAAGAACCCGGCGAAATATTTCATTGATGAGCC
GGTTGCTGTTCTCTCGAACAACCGTCCCGCTGGCTACATGGTCAGTGCCAAAGTGTTTGAAGAGCTGATCGACCTGTTGG
AAGAGAAGCAGGGCAGGGTTCATACCGCTGCGCGATTCAGGCCATCTGCTGAACGTTTAAGCGATATTGCGGATAGCGGG
CAGAAACTGCTGCAAAACGCCTCCGACAAGGATCTGACCGAGTTCACGGAATGA