Detailed information of TA system TA115649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1850276..1850936
• Replicon: chromosome
• Accession: NZ_CP034276
• Organism: Pectobacterium versatile strain 14A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A221T809
• Locus tag: EIP93_RS08260
• Protein ID: WP_044203475.1
• Coordinates: 1850276..1850629 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EIP93_RS08265
• Protein ID: WP_010298722.1
• Coordinates: 1850634..1850936 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EIP93_RS08240	1846590..1847846	+	1257	WP_103941182.1	multidrug effflux MFS transporter	-
EIP93_RS08245	1847892..1848731	-	840	WP_010298718.1	glutathione-dependent disulfide-bond oxidoreductase	-
EIP93_RS08250	1848895..1849506	-	612	WP_125232550.1	NAD(P)-dependent oxidoreductase	-
EIP93_RS08255	1849609..1850049	+	441	WP_125232551.1	helix-turn-helix transcriptional regulator	-
EIP93_RS08260	1850276..1850629	+	354	WP_044203475.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EIP93_RS08265	1850634..1850936	+	303	WP_010298722.1	XRE family transcriptional regulator	Antitoxin
EIP93_RS08270	1850974..1851978	-	1005	WP_125232552.1	threonine/serine dehydratase	-
EIP93_RS08275	1852031..1852369	+	339	WP_103862011.1	helix-turn-helix domain-containing protein	-
EIP93_RS08280	1852385..1852789	-	405	WP_206428390.1	winged helix-turn-helix transcriptional regulator	-
EIP93_RS08285	1852867..1853292	+	426	WP_039540880.1	DoxX family protein	-
EIP93_RS08290	1853412..1854047	+	636	WP_107333072.1	NAD(P)H-binding protein	-
EIP93_RS08295	1854115..1854708	-	594	WP_119158537.1	flavodoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13609.59 Da
• Isoelectric Point: 9.8031

>T115649 WP_044203475.1 NZ_CP034276:1850276-1850629 [Pectobacterium versatile]
VWRIETTDVFDSWFSALNDRDRACVLAALIVLREKGPMLSRPYADTVKGSRYSNMKELRIQSRGTPIRAFFAFDPHRTGI
LLCAGNKSGNEKRFYDEMLPFAERELTNYLKKSEKRE

Nucleotide

• Length: 354 bp

>T115649 NZ_CP034276:1850276-1850629 [Pectobacterium versatile]
GTGTGGAGAATTGAAACGACGGACGTGTTTGATAGCTGGTTCAGCGCCTTAAACGATAGGGATCGAGCTTGCGTGCTTGC
AGCACTTATCGTGTTGCGGGAAAAAGGTCCAATGTTATCTAGACCTTATGCCGATACGGTTAAAGGTTCTCGTTATAGCA
ACATGAAAGAGCTGCGTATTCAGAGCCGAGGAACGCCGATACGGGCATTTTTTGCCTTCGACCCACATCGTACAGGGATC
CTGCTCTGCGCGGGCAATAAGTCCGGCAATGAAAAGCGCTTTTACGATGAGATGCTCCCGTTTGCTGAGCGGGAGTTAAC
AAATTATCTGAAAAAATCAGAGAAGAGGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11282.16 Da
• Isoelectric Point: 5.6573

>AT115649 WP_010298722.1 NZ_CP034276:1850634-1850936 [Pectobacterium versatile]
MGRTLEQLLADEKPEVVVDAHAIATEMLLNIHLAELRERVQKTQVEMAQVLGLKQPTVAVMEKPGRDLKLSTLKRYVEAT
GSKLRLDVELPDGSHYEFML

Nucleotide

• Length: 303 bp

>AT115649 NZ_CP034276:1850634-1850936 [Pectobacterium versatile]
ATGGGCAGAACGCTGGAACAGCTTCTTGCGGATGAAAAACCAGAGGTTGTGGTCGATGCTCACGCTATAGCGACAGAGAT
GCTGCTCAATATTCATCTCGCTGAACTGCGTGAGAGGGTGCAAAAAACGCAAGTAGAAATGGCACAGGTGCTTGGGTTAA
AACAGCCGACCGTGGCGGTAATGGAGAAACCCGGCCGAGATCTGAAACTTTCCACGCTTAAACGCTATGTCGAAGCGACC
GGCAGTAAGCTACGTCTTGATGTTGAACTGCCGGACGGCTCGCATTACGAATTTATGCTGTAG

Structures

Toxin

• AlphaFold DB: A0A221T809