Detailed information of TA system TA115633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2478967..2479484
• Replicon: chromosome
• Accession: NZ_CP034259
• Organism: Staphylococcus aureus strain P1D12C1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: EFD74_RS12400
• Protein ID: WP_000113265.1
• Coordinates: 2478967..2479233 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: EFD74_RS12405
• Protein ID: WP_000584499.1
• Coordinates: 2479233..2479484 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFD74_RS12370	2474395..2474931	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
EFD74_RS12375	2475165..2475989	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
EFD74_RS12380	2476206..2476388	-	183	WP_000230292.1	hypothetical protein	-
EFD74_RS12385	2476472..2476939	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
EFD74_RS12390	2477125..2478672	-	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
EFD74_RS12395	2478861..2478932	-	72	Protein_2376	hypothetical protein	-
EFD74_RS12400	2478967..2479233	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
EFD74_RS12405	2479233..2479484	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EFD74_RS12410	2479649..2479750	-	102	Protein_2379	hypothetical protein	-
EFD74_RS12415	2479757..2480356	-	600	WP_000162810.1	DsbA family protein	-
EFD74_RS12420	2480375..2480737	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
EFD74_RS12425	2480992..2482242	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
EFD74_RS12430	2482339..2483070	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
EFD74_RS12435	2483067..2483786	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T115633 WP_000113265.1 NZ_CP034259:c2479233-2478967 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T115633 NZ_CP034259:c2479233-2478967 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT115633 WP_000584499.1 NZ_CP034259:c2479484-2479233 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT115633 NZ_CP034259:c2479484-2479233 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60