Detailed information of TA system TA115585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 1670844..1671477
• Replicon: chromosome
• Accession: NZ_CP034240
• Organism: Aliivibrio salmonicida strain VS224

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1E5D3W9
• Locus tag: EIJ81_RS09970
• Protein ID: WP_023603298.1
• Coordinates: 1671145..1671477 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EIJ81_RS09965
• Protein ID: WP_012550279.1
• Coordinates: 1670844..1671158 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EIJ81_RS09930	1666858..1667133	+	276	WP_044583260.1	DUF1778 domain-containing protein	-
EIJ81_RS09935	1667130..1667633	+	504	WP_012550273.1	GNAT family N-acetyltransferase	-
EIJ81_RS09940	1667922..1668167	-	246	WP_012550274.1	hypothetical protein	-
EIJ81_RS09945	1668157..1668420	-	264	WP_012550275.1	BrnT family toxin	-
EIJ81_RS09950	1668600..1669718	-	1119	WP_012550276.1	hypothetical protein	-
EIJ81_RS09955	1669711..1670019	-	309	WP_012550277.1	hypothetical protein	-
EIJ81_RS09960	1670001..1670663	-	663	WP_012550278.1	hypothetical protein	-
EIJ81_RS09965	1670844..1671158	-	315	WP_012550279.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
EIJ81_RS09970	1671145..1671477	-	333	WP_023603298.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EIJ81_RS09975	1671686..1672135	-	450	WP_044583261.1	hypothetical protein	-
EIJ81_RS09980	1672300..1672755	-	456	WP_012550282.1	hypothetical protein	-
EIJ81_RS09985	1672871..1673617	-	747	WP_012550285.1	hypothetical protein	-
EIJ81_RS09990	1673737..1674618	-	882	WP_012548944.1	IS982-like element ISVsa6 family transposase	-
EIJ81_RS09995	1674771..1675047	+	277	Protein_1599	transposase	-
EIJ81_RS10000	1675128..1676321	+	1194	WP_012548955.1	IS91-like element ISVsa9 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	1668157..1673359	5202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12934.81 Da
• Isoelectric Point: 10.0960

>T115585 WP_023603298.1 NZ_CP034240:c1671477-1671145 [Aliivibrio salmonicida]
MKSIFVESTIFEKYRNEYLSDEEFRLFQAELMSNPKQGDVIQGTGGLRKIRVASKGKGKRGGSRVIYYFLDEKRRFYLLT
IYGKNEMSDLTADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T115585 NZ_CP034240:c1671477-1671145 [Aliivibrio salmonicida]
ATGAAAAGTATATTTGTCGAATCGACCATATTTGAAAAGTACCGTAATGAGTATCTCAGCGATGAAGAGTTTAGACTTTT
TCAAGCTGAATTAATGTCTAATCCGAAACAGGGTGATGTGATTCAAGGTACAGGTGGTTTGCGAAAGATTCGAGTTGCAA
GTAAAGGCAAGGGCAAGCGTGGTGGCTCTCGTGTTATCTATTACTTCCTCGATGAAAAACGTCGTTTCTACTTGCTGACA
ATTTATGGCAAGAATGAAATGTCCGATTTAACCGCAGACCAAAAGAAACAATTAAAGGCTTTTATGGAGGCGTGGCGTAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11763.20 Da
• Isoelectric Point: 6.2155

>AT115585 WP_012550279.1 NZ_CP034240:c1671158-1670844 [Aliivibrio salmonicida]
MSNRDLFTELSSAIIEAKEHSEGKLTLKTHQVNDFSELDISPNEIVSIREQFNMSRGVFAHLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT115585 NZ_CP034240:c1671158-1670844 [Aliivibrio salmonicida]
ATGAGCAATCGTGACCTATTTACAGAGTTAAGTTCAGCTATTATTGAGGCTAAAGAACATTCTGAGGGTAAGTTAACCCT
TAAAACTCATCAAGTTAACGATTTCAGTGAGTTAGATATTTCACCTAATGAAATCGTGAGTATTCGTGAACAATTTAATA
TGTCTCGTGGTGTGTTTGCACATTTACTGCATACATCATCTCGTACATTAGAAAATTGGGAACAAGGGCGTAGCGCACCA
AATGGACAAGCGGTAACCCTTTTAAAATTGGTTCAGCGTCATCCAGAAACCCTGTCACACATCGCTGAGCTATAA

Structures

Toxin

• AlphaFold DB: A0A1E5D3W9