Detailed information of TA system TA115539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1777418..1778078
• Replicon: chromosome
• Accession: NZ_CP034225
• Organism: Kosakonia sp. CCTCC M2018092

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EH164_RS08320
• Protein ID: WP_124967901.1
• Coordinates: 1777418..1777783 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EH164_RS08325
• Protein ID: WP_042717293.1
• Coordinates: 1777776..1778078 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH164_RS08300	1772628..1773368	+	741	WP_124967898.1	adenosylcobinamide-GDP ribazoletransferase	-
EH164_RS08305	1773388..1774443	+	1056	WP_124967899.1	nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase	-
EH164_RS08310	1774544..1774855	-	312	WP_023479098.1	helix-turn-helix domain-containing protein	-
EH164_RS08315	1775074..1777176	+	2103	WP_124967900.1	elongation factor G	-
EH164_RS08320	1777418..1777783	+	366	WP_124967901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EH164_RS08325	1777776..1778078	+	303	WP_042717293.1	helix-turn-helix transcriptional regulator	Antitoxin
EH164_RS08330	1778142..1779098	+	957	WP_124971353.1	L,D-transpeptidase	-
EH164_RS08335	1779221..1780246	+	1026	WP_124967902.1	LLM class flavin-dependent oxidoreductase	-
EH164_RS08340	1780614..1781471	+	858	WP_124967903.1	glucose 1-dehydrogenase	-
EH164_RS08345	1781488..1782552	+	1065	WP_124967904.1	class I mannose-6-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14033.20 Da
• Isoelectric Point: 9.0405

>T115539 WP_124967901.1 NZ_CP034225:1777418-1777783 [Kosakonia sp. CCTCC M2018092]
VWQVEMTETFDEWFFTLGERERASVLAGLMVLKVKGPLLPRPYADTVKGSRYPNMKELRVQSNGQPLRIFFAFDPSRKGI
LLCAGNKAGYDKRFYEVMIPVADREFTLHLQKNDKRSECNE

Nucleotide

• Length: 366 bp

>T115539 NZ_CP034225:1777418-1777783 [Kosakonia sp. CCTCC M2018092]
GTGTGGCAGGTTGAAATGACAGAAACGTTTGATGAGTGGTTTTTTACACTGGGTGAGCGCGAACGCGCCAGCGTGCTGGC
GGGCCTGATGGTGCTCAAGGTAAAAGGCCCGCTGTTGCCGAGGCCCTACGCCGATACGGTAAAAGGTTCTCGCTATCCCA
ATATGAAAGAGCTGCGTGTGCAGAGCAATGGCCAGCCGTTACGCATATTTTTCGCCTTCGATCCCTCCCGCAAAGGGATT
CTGCTCTGTGCAGGAAACAAGGCGGGTTATGACAAGCGTTTTTACGAGGTGATGATCCCTGTCGCTGATCGTGAGTTTAC
GCTGCATCTGCAGAAGAACGACAAAAGGAGTGAATGCAATGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11261.04 Da
• Isoelectric Point: 6.4730

>AT115539 WP_042717293.1 NZ_CP034225:1777776-1778078 [Kosakonia sp. CCTCC M2018092]
MSKTLEQLIAEQTPEVVAKAQTLAQEMLLNIHLAELRERVQKTQNEMAQELGLKQPTVAVMEKQGRDIKLSTLKRYVEAT
GSKLRIDIELPDGSHYGFAL

Nucleotide

• Length: 303 bp

>AT115539 NZ_CP034225:1777776-1778078 [Kosakonia sp. CCTCC M2018092]
ATGAGTAAAACACTCGAGCAATTAATTGCCGAACAGACACCTGAGGTTGTCGCGAAGGCGCAAACCCTGGCGCAGGAGAT
GCTGCTTAATATTCACCTTGCTGAGCTGCGCGAGCGCGTGCAAAAAACGCAGAACGAAATGGCGCAGGAGCTGGGGCTTA
AGCAGCCCACCGTGGCTGTGATGGAGAAGCAGGGGAGAGATATTAAGTTATCGACGCTGAAACGCTACGTCGAGGCGACG
GGGAGCAAGCTGCGCATCGATATCGAGTTGCCCGACGGCTCCCATTATGGTTTCGCGCTCTGA