Detailed information of TA system TA115535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 49315..50022
• Replicon: chromosome
• Accession: NZ_CP034225
• Organism: Kosakonia sp. CCTCC M2018092

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: EH164_RS00250
• Protein ID: WP_124966952.1
• Coordinates: 49717..50022 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: -
• Locus tag: EH164_RS00245
• Protein ID: WP_124966951.1
• Coordinates: 49315..49716 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH164_RS00225	45091..46038	+	948	WP_042715135.1	ABC transporter permease	-
EH164_RS00230	46035..47018	+	984	WP_042715059.1	ABC transporter ATP-binding protein	-
EH164_RS00235	47015..47989	+	975	WP_124966950.1	ATP-binding cassette domain-containing protein	-
EH164_RS00240	48063..49256	+	1194	WP_042715056.1	glutathione-dependent formaldehyde dehydrogenase	-
EH164_RS00245	49315..49716	-	402	WP_124966951.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
EH164_RS00250	49717..50022	-	306	WP_124966952.1	type II toxin-antitoxin system MqsR family toxin	Toxin
EH164_RS00255	50180..50833	-	654	WP_124966953.1	oxygen-insensitive NAD(P)H nitroreductase	-
EH164_RS00260	51028..51963	+	936	WP_124971187.1	LysR family transcriptional regulator	-
EH164_RS00265	52025..52978	+	954	WP_124966954.1	aldo/keto reductase	-
EH164_RS00270	53101..54039	+	939	WP_124966955.1	peptidoglycan-binding protein	-
EH164_RS00275	54036..54464	+	429	WP_124966956.1	DUF1311 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-127)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11560.57 Da
• Isoelectric Point: 8.9492

>T115535 WP_124966952.1 NZ_CP034225:c50022-49717 [Kosakonia sp. CCTCC M2018092]
MEKGTPHVRLHIVKRMVLEGKVRTTASAVAGAIELGFNPPPFDEMCAVILALTSEDFFKSMTAYADHTVWHDVYRPVWKR
QKLYLKLIVSDDVLIVSFKER

Nucleotide

• Length: 306 bp

>T115535 NZ_CP034225:c50022-49717 [Kosakonia sp. CCTCC M2018092]
ATGGAGAAAGGAACTCCCCATGTCCGCTTACATATCGTGAAGCGGATGGTGCTCGAGGGAAAGGTTAGAACCACAGCCAG
CGCTGTCGCGGGAGCGATTGAATTGGGCTTTAATCCACCGCCATTTGACGAGATGTGCGCGGTGATTCTGGCGCTCACTT
CAGAAGATTTTTTTAAAAGCATGACGGCATATGCCGATCATACGGTGTGGCACGATGTTTACCGACCAGTCTGGAAGAGA
CAAAAACTCTATCTGAAGTTAATCGTCTCTGACGATGTACTGATCGTGTCGTTTAAGGAGAGATAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14933.12 Da
• Isoelectric Point: 8.4858

>AT115535 WP_124966951.1 NZ_CP034225:c49716-49315 [Kosakonia sp. CCTCC M2018092]
MKCPVCGAAGLKHDTRNIEYRYKGKVTTLTAIAGDYCDACGEIIFDRLNGEHYSKQVAAFVRKVNAEEVDPQFIATIRKR
FNLDQRQAAEMFGGGANAFSRYETGRVAPPRPLVLLFKALDKHPELFEELKHA

Nucleotide

• Length: 402 bp

>AT115535 NZ_CP034225:c49716-49315 [Kosakonia sp. CCTCC M2018092]
ATGAAATGCCCTGTTTGCGGCGCTGCGGGACTCAAACATGACACCCGCAACATTGAATACCGTTACAAAGGGAAAGTGAC
TACGCTCACTGCGATTGCAGGCGATTACTGCGACGCATGTGGAGAGATCATTTTCGACCGTCTGAACGGTGAACATTACT
CAAAACAGGTTGCAGCTTTTGTCCGCAAAGTTAACGCCGAAGAAGTTGATCCTCAGTTTATTGCCACCATCCGAAAGCGG
TTTAATCTCGATCAGCGCCAGGCGGCAGAGATGTTTGGCGGCGGCGCAAACGCCTTTAGCCGGTACGAAACCGGGCGTGT
TGCCCCGCCAAGACCTTTAGTTTTGCTCTTTAAGGCGCTGGACAAGCATCCAGAGCTTTTCGAGGAGCTAAAACATGCCT
GA