Detailed information of TA system TA115531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3595842..3596641
• Replicon: chromosome
• Accession: NZ_CP034213
• Organism: Escherichia albertii strain NCTC 9362

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: DSB70_RS18430
• Protein ID: WP_125060402.1
• Coordinates: 3595842..3596306 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: DSB70_RS18435
• Protein ID: WP_001296435.1
• Coordinates: 3596306..3596641 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSB70_RS18400 (3590843)	3590843..3591277	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
DSB70_RS18405 (3591295)	3591295..3592173	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
DSB70_RS18410 (3592163)	3592163..3592942	-	780	WP_000406226.1	PTS N-acetylgalactosamine transporter subunit IIC	-
DSB70_RS18415 (3592953)	3592953..3593426	-	474	Protein_3579	PTS N-acetylgalactosamine transporter subunit IIB	-
DSB70_RS18420 (3593449)	3593449..3594729	-	1281	WP_125060400.1	tagatose-bisphosphate aldolase subunit KbaZ	-
DSB70_RS18425 (3594978)	3594978..3595787	+	810	WP_125060401.1	aga operon transcriptional regulator AgaR	-
DSB70_RS18430 (3595842)	3595842..3596306	-	465	WP_125060402.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
DSB70_RS18435 (3596306)	3596306..3596641	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
DSB70_RS18440 (3596790)	3596790..3598361	-	1572	WP_125060403.1	galactarate dehydratase	-
DSB70_RS18445 (3598732)	3598732..3600066	+	1335	WP_125060404.1	galactarate/glucarate/glycerate transporter GarP	-
DSB70_RS18450 (3600082)	3600082..3600852	+	771	WP_001058056.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17849.29 Da
• Isoelectric Point: 9.7301

>T115531 WP_125060402.1 NZ_CP034213:c3596306-3595842 [Escherichia albertii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRFRLFFRYSEKEKVIILGWMNDENTLRTYSKKTDAYTVFSKMLKRGHPPADWETLTQETEEKH

Nucleotide

• Length: 465 bp

>T115531 NZ_CP034213:c3596306-3595842 [Escherichia albertii]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAACCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTTTCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACACTGCGCACCTACAGTAAAAAAACAGATGCCTATACAGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACTCTCACCCAAGAGACAGAAGAAAAGCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT115531 WP_001296435.1 NZ_CP034213:c3596641-3596306 [Escherichia albertii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT115531 NZ_CP034213:c3596641-3596306 [Escherichia albertii]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: S1PPV5