Detailed information of TA system TA115524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2014433..2015083
• Replicon: chromosome
• Accession: NZ_CP034213
• Organism: Escherichia albertii strain NCTC 9362

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DSB70_RS10470
• Protein ID: WP_059224977.1
• Coordinates: 2014742..2015083 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A3T5VD61
• Locus tag: DSB70_RS10465
• Protein ID: WP_025237546.1
• Coordinates: 2014433..2014732 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSB70_RS10435 (2009461)	2009461..2009772	-	312	WP_000410252.1	hypothetical protein	-
DSB70_RS10440 (2009777)	2009777..2010169	-	393	WP_000273381.1	flagellar export chaperone FliS	-
DSB70_RS10445 (2010192)	2010192..2011508	-	1317	WP_000609691.1	flagellar filament capping protein FliD	-
DSB70_RS23745 (2011575)	2011575..2011901	-	327	WP_241201543.1	hypothetical protein	-
DSB70_RS10455 (2012123)	2012123..2013037	-	915	WP_125059914.1	lateral flagellin LafA	-
DSB70_RS10460 (2013524)	2013524..2014375	+	852	WP_125059915.1	winged helix-turn-helix domain-containing protein	-
DSB70_RS10465 (2014433)	2014433..2014732	-	300	WP_025237546.1	XRE family transcriptional regulator	Antitoxin
DSB70_RS10470 (2014742)	2014742..2015083	-	342	WP_059224977.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DSB70_RS10475 (2015159)	2015159..2016136	-	978	WP_125059916.1	flagellar hook-associated protein	-
DSB70_RS10480 (2016153)	2016153..2017082	-	930	WP_125059917.1	flagellar hook-associated protein FlgL	-
DSB70_RS10485 (2017097)	2017097..2018473	-	1377	WP_125059918.1	flagellar hook-associated protein FlgK	-
DSB70_RS10490 (2019031)	2019031..2019330	-	300	WP_059254376.1	rod-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1999933..2023215	23282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13017.79 Da
• Isoelectric Point: 5.7334

>T115524 WP_059224977.1 NZ_CP034213:c2015083-2014742 [Escherichia albertii]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRNHLKK

Nucleotide

• Length: 342 bp

>T115524 NZ_CP034213:c2015083-2014742 [Escherichia albertii]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTGCTTGC
TGCAATGCTGATTCTTGCAGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGACACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCAGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAATCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10962.78 Da
• Isoelectric Point: 9.7665

>AT115524 WP_025237546.1 NZ_CP034213:c2014732-2014433 [Escherichia albertii]
MATLKELMAKQSPESQARIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRVAFQI

Nucleotide

• Length: 300 bp

>AT115524 NZ_CP034213:c2014732-2014433 [Escherichia albertii]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGCGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACCCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTCGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCTAATGGTAAGAGAGTTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A3T5VD61