Detailed information of TA system TA115431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 2237229..2237941
• Replicon: chromosome
• Accession: NZ_CP034166
• Organism: Escherichia albertii strain 2014C-4015

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U8WCQ7
• Locus tag: AYR02_RS12140
• Protein ID: WP_000162413.1
• Coordinates: 2237229..2237531 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AYR02_RS12145
• Protein ID: WP_000806446.1
• Coordinates: 2237603..2237941 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR02_RS12120	2234091..2234933	+	843	WP_095576108.1	23S rRNA (adenine(2030)-N(6))-methyltransferase	-
AYR02_RS12125	2235005..2236357	+	1353	WP_054410173.1	glutathione-disulfide reductase	-
AYR02_RS12130	2236411..2236494	-	84	WP_001295215.1	hypothetical protein	-
AYR02_RS12135	2236586..2237065	-	480	WP_124782641.1	hypothetical protein	-
AYR02_RS12140	2237229..2237531	+	303	WP_000162413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYR02_RS12145	2237603..2237941	+	339	WP_000806446.1	HigA family addiction module antidote protein	Antitoxin
AYR02_RS12150	2237998..2238177	+	180	WP_002460167.1	hypothetical protein	-
AYR02_RS12155	2238518..2239084	+	567	WP_001044206.1	outer membrane lipoprotein Slp	-
AYR02_RS12160	2239231..2239761	+	531	WP_000480294.1	LuxR family transcriptional regulator	-
AYR02_RS12165	2239833..2240861	-	1029	WP_001110409.1	hematinate-forming heme oxygenase ChuS	-
AYR02_RS12170	2240910..2242892	-	1983	WP_124782642.1	TonB-dependent heme/hemoglobin receptor ChuA/ShuA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11871.59 Da
• Isoelectric Point: 9.8664

>T115431 WP_000162413.1 NZ_CP034166:2237229-2237531 [Escherichia albertii]
MTKKINIKDFRDAWLDDFFEFSTPHKKIPSDIHITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNNQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T115431 NZ_CP034166:2237229-2237531 [Escherichia albertii]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACACAAAAA
GATACCTTCTGATATTCATATTACATTGTTGCGGAAGCTGGATATTATCAATGCCGCAACTACCTGGAAGGACTTGAGAT
CACCACCAGGTAATCGGTATGAGGAATTGTCAGGGAAGCTAAACGGCTATTCATCAATAAGGGTGAATAATCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAAGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13037.92 Da
• Isoelectric Point: 5.2075

>AT115431 WP_000806446.1 NZ_CP034166:2237603-2237941 [Escherichia albertii]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRITTAEEFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT115431 NZ_CP034166:2237603-2237941 [Escherichia albertii]
ATGAAACAGGCAACCAGAAAACCGACGACTGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTTAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGCAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGACCTGTGG
GAAGTTGAAAATGATATGCGCGTTCAGGAAGAGTTAAGCCGTATCACTACTGCTGAAGAATTTATTTCTCAGCGGAATCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U8WCQ7