Detailed information of TA system TA115403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2565673..2566367
• Replicon: chromosome
• Accession: NZ_CP034162
• Organism: Escherichia albertii strain 06-3542

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: CCS91_RS12980
• Protein ID: WP_001263500.1
• Coordinates: 2565673..2566071 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: CCS91_RS12985
• Protein ID: WP_000554758.1
• Coordinates: 2566074..2566367 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCS91_RS12950	2561981..2562439	-	459	WP_001291989.1	xanthine phosphoribosyltransferase	-
CCS91_RS12955	2562700..2564157	+	1458	WP_001292996.1	cytosol nonspecific dipeptidase	-
CCS91_RS12960	2564216..2564458	+	243	WP_059222190.1	hypothetical protein	-
CCS91_RS12970	2564599..2565198	-	600	WP_072249376.1	peptide chain release factor H	-
CCS91_RS12975	2565211..2565663	-	453	WP_095575504.1	GNAT family N-acetyltransferase	-
CCS91_RS12980	2565673..2566071	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CCS91_RS12985	2566074..2566367	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CCS91_RS12990	2566419..2567474	-	1056	WP_059217800.1	DNA polymerase IV	-
CCS91_RS12995	2567604..2568371	-	768	WP_072243690.1	putative lateral flagellar export/assembly protein LafU	-
CCS91_RS13000	2568343..2570055	+	1713	Protein_2480	flagellar biosynthesis protein FlhA	-
CCS91_RS13005	2570171..2570668	-	498	WP_059275984.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T115403 WP_001263500.1 NZ_CP034162:c2566071-2565673 [Escherichia albertii]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T115403 NZ_CP034162:c2566071-2565673 [Escherichia albertii]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT115403 WP_000554758.1 NZ_CP034162:c2566367-2566074 [Escherichia albertii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT115403 NZ_CP034162:c2566367-2566074 [Escherichia albertii]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3