Detailed information of TA system TA115381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 342388..343106
• Replicon: plasmid pPagL15_1
• Accession: NZ_CP034149
• Organism: Pantoea agglomerans strain L15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CBF16_RS20655
• Protein ID: WP_010247047.1
• Coordinates: 342795..343106 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CBF16_RS20650
• Protein ID: WP_010247048.1
• Coordinates: 342388..342798 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBF16_RS20630	338161..339168	+	1008	WP_010670390.1	putative FMN-dependent luciferase-like monooxygenase	-
CBF16_RS20635	339165..340265	+	1101	WP_124890599.1	alkylhydroperoxidase domain protein	-
CBF16_RS20640	340268..341407	+	1140	WP_010670392.1	amidohydrolase	-
CBF16_RS20645	341428..342273	+	846	WP_124890600.1	VOC family protein	-
CBF16_RS20650	342388..342798	-	411	WP_010247048.1	helix-turn-helix domain-containing protein	Antitoxin
CBF16_RS20655	342795..343106	-	312	WP_010247047.1	type II toxin-antitoxin system HigB family toxin	Toxin
CBF16_RS20660	343464..345686	-	2223	WP_124890601.1	ferric-rhodotorulic acid/ferric-coprogen receptor FhuE	-
CBF16_RS20665	346146..346700	+	555	WP_045140216.1	lipid IV(A) palmitoyltransferase PagP	-
CBF16_RS20670	346719..347825	-	1107	WP_124890602.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..583567	583567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12112.98 Da
• Isoelectric Point: 9.6250

>T115381 WP_010247047.1 NZ_CP034149:c343106-342795 [Pantoea agglomerans]
MHVISRVPFDEAASKYPNAAAALNLLYRHLRQGDFSGPDDLKALYASLDRMKYRAKWWVIDVSGNQLRVMFFADFERSKI
FIKHICTHAEYDKLVKKYREVTE

Nucleotide

• Length: 312 bp

>T115381 NZ_CP034149:c343106-342795 [Pantoea agglomerans]
ATGCATGTTATTTCACGTGTGCCGTTTGATGAGGCGGCCAGCAAATATCCTAATGCAGCGGCCGCACTTAATCTGCTTTA
TCGCCATCTCAGGCAAGGCGATTTCAGTGGTCCTGACGATCTTAAAGCGCTTTATGCAAGCCTTGATCGTATGAAATATC
GTGCTAAATGGTGGGTCATCGACGTGAGTGGCAACCAGTTGCGCGTGATGTTCTTCGCGGATTTTGAGCGAAGTAAAATC
TTTATTAAGCATATCTGCACGCACGCTGAGTACGACAAGCTTGTTAAAAAATATCGGGAGGTTACCGAATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15289.30 Da
• Isoelectric Point: 4.5110

>AT115381 WP_010247048.1 NZ_CP034149:c342798-342388 [Pantoea agglomerans]
MNTEAIRLSNELAEAVPLLAGSSQREDYEEALKLVEYLIEHEPDNPLVDLLCARIAHYEDKDPELAEFNARQNEISTGPG
ILRVLIEQHNLSLSDFENEIGKKSLVSRILSGERQLTVSHIRALCARFGLSPSLFF

Nucleotide

• Length: 411 bp

>AT115381 NZ_CP034149:c342798-342388 [Pantoea agglomerans]
ATGAATACTGAAGCTATCAGGCTGTCAAATGAGCTCGCTGAAGCGGTACCGCTGCTGGCTGGCAGCAGCCAGCGCGAGGA
TTATGAAGAAGCACTGAAACTGGTCGAATATCTGATTGAACATGAGCCGGATAATCCGCTGGTTGACCTGCTTTGCGCGC
GCATTGCTCACTATGAAGATAAAGATCCGGAGCTGGCCGAATTTAATGCACGTCAAAATGAAATCAGCACAGGCCCCGGC
ATTCTGCGCGTGCTTATTGAGCAACACAATCTTAGCCTGAGCGACTTCGAAAATGAGATCGGTAAAAAATCGCTGGTTAG
CCGAATCCTCAGCGGCGAACGTCAGTTGACTGTGTCGCATATCCGGGCATTATGCGCCCGGTTTGGCCTGTCGCCATCTC
TGTTTTTCTGA