Detailed information of TA system TA115378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2500876..2501536
• Replicon: chromosome
• Accession: NZ_CP034148
• Organism: Pantoea agglomerans strain L15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A349IFT5
• Locus tag: CBF16_RS11945
• Protein ID: WP_010670918.1
• Coordinates: 2501183..2501536 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CBF16_RS11940
• Protein ID: WP_010247918.1
• Coordinates: 2500876..2501178 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBF16_RS11920	2496715..2497740	-	1026	WP_170933484.1	ABC transporter permease	-
CBF16_RS11925	2497764..2499248	-	1485	WP_010247905.1	sugar ABC transporter ATP-binding protein	-
CBF16_RS11930	2499281..2500204	-	924	WP_010247907.1	sugar ABC transporter substrate-binding protein	-
CBF16_RS11940	2500876..2501178	-	303	WP_010247918.1	XRE family transcriptional regulator	Antitoxin
CBF16_RS11945	2501183..2501536	-	354	WP_010670918.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CBF16_RS11950	2501835..2502923	-	1089	WP_124890088.1	hypothetical protein	-
CBF16_RS11955	2503319..2505481	+	2163	WP_124890089.1	ferric-rhodotorulic acid/ferric-coprogen receptor FhuE	-
CBF16_RS11960	2505558..2505959	-	402	WP_010247930.1	hypothetical protein	-
CBF16_RS11965	2506058..2506339	-	282	WP_124890090.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13649.71 Da
• Isoelectric Point: 8.4949

>T115378 WP_010670918.1 NZ_CP034148:c2501536-2501183 [Pantoea agglomerans]
VWMIKTTERFDRWFTLLNESDRACVLAALMVLREKGPGLSRPYADTLKGSVYINMKELRIQSRGDPIRAFFAFDPNRTAI
VLCAGNKVGNEKRFYQEMLPVADREFTHWLNSFKDEE

Nucleotide

• Length: 354 bp

>T115378 NZ_CP034148:c2501536-2501183 [Pantoea agglomerans]
GTGTGGATGATTAAAACGACGGAGAGGTTTGATCGCTGGTTTACCTTGCTTAATGAGAGCGATCGCGCCTGCGTACTGGC
AGCATTAATGGTGTTGCGCGAAAAGGGGCCGGGGTTGTCCAGGCCATACGCGGATACGCTAAAAGGCTCTGTTTATATCA
ACATGAAAGAGCTGCGTATTCAGAGTCGCGGCGATCCGATAAGGGCTTTCTTTGCTTTTGACCCAAACCGAACGGCGATT
GTGCTTTGTGCCGGAAATAAGGTGGGCAATGAAAAGCGTTTTTATCAGGAGATGCTTCCGGTTGCCGATCGGGAATTCAC
ACACTGGCTGAATAGTTTTAAAGATGAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10941.72 Da
• Isoelectric Point: 5.7251

>AT115378 WP_010247918.1 NZ_CP034148:c2501178-2500876 [Pantoea agglomerans]
MGRTLEQLIADEKPEVVADAQAMATDILLNIHLAELREKVQKTQVEMAKALGITQPTVAGMEKPGRDLKLSTLKRYIDAA
GGKLRLVVELPDGSHYGFEV

Nucleotide

• Length: 303 bp

>AT115378 NZ_CP034148:c2501178-2500876 [Pantoea agglomerans]
ATGGGCAGAACGCTTGAACAGCTTATTGCGGATGAAAAACCGGAAGTCGTTGCCGACGCACAGGCGATGGCGACGGATAT
CCTGCTCAACATTCACCTTGCCGAGCTGCGCGAGAAAGTGCAGAAAACACAGGTCGAAATGGCAAAGGCGCTGGGCATTA
CACAGCCTACCGTGGCGGGTATGGAGAAGCCGGGCCGCGATCTGAAGCTCTCCACGCTCAAGCGCTATATCGATGCGGCA
GGGGGTAAGCTGCGGCTGGTGGTGGAACTGCCGGACGGCTCGCACTACGGGTTTGAGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A349IFT5